{"id":"W70110460","doi":"10.1007/978-1-60761-533-0_2","title":"New Protocol for Lentiviral Vector Mass Production","year":2009,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Biotechnology Research Institute","funders":"","keywords":"Transfection; Viral vector; Polyethylenimine; Chemistry; Chromatography; HEK 293 cells; Cell culture; Molecular biology; Biology; Recombinant DNA; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001265588,0.0002150591,0.0002369768,0.0001336035,0.00004952207,0.00001629413,0.0003185033,0.0002881582,0.00002941657],"category_scores_gemma":[0.0004656565,0.0002045014,0.000142592,0.000208139,0.00006812459,0.00000333121,0.00004857287,0.0001618529,0.000004479711],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003455488,"about_ca_system_score_gemma":0.0001737846,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006727571,"about_ca_topic_score_gemma":0.00001858428,"domain_scores_codex":[0.9976343,0.0008394845,0.000291157,0.0006664365,0.00008303322,0.0004855712],"domain_scores_gemma":[0.9992018,0.00002361176,0.00008232045,0.00052535,0.00008283343,0.00008412353],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001959456,0.00005948139,0.0001185495,0.00001211,0.00002460497,0.000002006439,0.00001002854,0.00005126025,0.8986083,0.0003445039,0.0005697271,0.09823995],"study_design_scores_gemma":[0.00139155,0.004675812,0.0001094084,0.00000856881,0.000005221374,0.000005298257,0.000004969021,0.00004616728,0.9458119,0.01034606,0.03737523,0.0002198738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.00567146,0.0001196499,0.9502566,0.000952203,0.0001644173,0.04241798,0.000004750479,0.00002409607,0.0003887833],"genre_scores_gemma":[0.002243177,0.000007458711,0.9456123,0.0005868254,0.0004738269,0.0498081,0.00006314412,0.00004388846,0.001161349],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.09802008,"threshold_uncertainty_score":0.8339324,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04610272399432718,"score_gpt":0.4708536701154399,"score_spread":0.4247509461211127,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}