{"id":"W70110460","doi":"10.1007/978-1-60761-533-0_2","title":"New Protocol for Lentiviral Vector Mass Production","year":2009,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":45,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Biotechnology Research Institute","funders":"","keywords":"Transfection; Viral vector; Polyethylenimine; Chemistry; Chromatography; HEK 293 cells; Cell culture; Molecular biology; Biology; Recombinant DNA; Biochemistry; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001728387,0.002057465,0.001660652,0.003394669,0.001519039,0.0009951243,0.002552094,0.001407583,0.02661388],"category_scores_gemma":[0.001070213,0.001488266,0.001465636,0.002051159,0.0006440927,0.000978543,0.001118199,0.004680801,0.02999958],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006518168,"about_ca_system_score_gemma":0.001047857,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006254272,"about_ca_topic_score_gemma":0.001385074,"domain_scores_codex":[0.9980111,0.0002909146,0.0002802939,0.0003962322,0.0008377579,0.0001837886],"domain_scores_gemma":[0.999398,0.000120688,0.00004294226,0.0001672614,0.0002005028,0.00007061155],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004012799,0.0005699313,0.0004129815,0.001708607,0.0001353165,0.0009325643,0.0004267473,0.0006146269,0.8017651,0.005946472,0.06383762,0.1232489],"study_design_scores_gemma":[0.0001388644,0.0004601485,0.00128503,0.0001865071,0.0001298501,0.002652444,0.00003374597,0.00142794,0.2946782,0.001610887,0.6972433,0.0001530883],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.02108448,0.02218014,0.831252,0.001386842,0.004137437,0.01371825,0.0258038,0.01465958,0.06577747],"genre_scores_gemma":[0.0359823,0.01938947,0.6996061,0.001471336,0.0006003287,0.02619179,0.09320541,0.002591974,0.1209613],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.02661388,"threshold_uncertainty_score":0.08903223,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04610272399432718,"score_gpt":0.4708536701154399,"score_spread":0.4247509461211127,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}