{"id":"W7108603298","doi":"10.1016/j.synbio.2025.11.014","title":"Efficient whole-cell biocatalytic synthesis of 2′-deoxy-2′-fluoroadenosine, a key building block for nucleic acid drugs","year":2025,"lang":"en","type":"article","venue":"Synthetic and Systems Biotechnology","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Natural Science Foundation of Shandong Province; Chinese Academy of Sciences; Taishan Scholar Foundation of Shandong Province; National Natural Science Foundation of China; Canadian Anesthesiologists' Society","keywords":"Purine nucleoside phosphorylase; Yield (engineering); Nucleic acid; Deamination; Substrate (aquarium); Nucleoside; Catalysis; Oligonucleotide","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001893906,0.0005279465,0.0003427534,0.0001406057,0.0001316466,0.0002985027,0.0002894737,0.0003743166,0.0003229466],"category_scores_gemma":[0.0001405716,0.0001473431,0.0003113184,0.0001650365,0.0001296221,0.0002341157,0.0002678784,0.000454989,0.0003306233],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000264357,"about_ca_system_score_gemma":0.0003091413,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008841766,"about_ca_topic_score_gemma":0.001551922,"domain_scores_codex":[0.9998734,0.00001227954,0.000009655903,0.00004358606,0.00003486413,0.00002625831],"domain_scores_gemma":[0.9999526,0.000006294611,0.00001351597,0.000007100492,0.0000114205,0.000009109529],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001082679,0.00001253374,0.00004659597,0.00003715908,0.00000374579,0.00002127024,0.000007312348,0.00007366998,0.9979897,0.00006607811,0.00003241966,0.00169878],"study_design_scores_gemma":[0.000002388417,0.00005372409,0.0003545063,0.000002609992,0.000007539785,0.00007408087,0.000006466105,0.0007985488,0.9970251,0.00001441329,0.001656946,0.000003817866],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9105144,0.005827321,0.0782446,0.0001989368,0.0001083537,0.0001085237,0.0009662167,0.0005564781,0.003475215],"genre_scores_gemma":[0.9583246,0.002672899,0.03575982,0.00004714683,0.0000103854,0.00005687097,0.000965731,0.00004961983,0.002112944],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008841766,"threshold_uncertainty_score":0.001918077,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00587312817590985,"score_gpt":0.2382942085631098,"score_spread":0.2324210803871999,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}