{"id":"W7110306144","doi":"10.64898/2025.12.02.691602","title":"A guide RNA repeat checkpoint steers CRISPR-Cas9 catalysis","year":2025,"lang":"en","type":"article","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Canada's Michael Smith Genome Sciences Centre; Michael Smith Health Research BC; McGill University","funders":"","keywords":"Cas9; RNA; Guide RNA; CRISPR; Mechanism (biology); RNA editing; Gene; Nucleic acid structure","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001680251,0.0002651067,0.0002582569,0.00009370139,0.0001643852,0.0004443348,0.0003901571,0.000379091,0.0008528605],"category_scores_gemma":[0.0002452041,0.0001671936,0.000149735,0.00007067673,0.0002441902,0.0002756405,0.0003969415,0.0004970128,0.0004490906],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000269284,"about_ca_system_score_gemma":0.0002379779,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003927448,"about_ca_topic_score_gemma":0.0004839581,"domain_scores_codex":[0.9998434,0.00001279426,0.00001005943,0.00006142011,0.00004637237,0.0000260283],"domain_scores_gemma":[0.999909,0.0000155208,0.00002564357,0.00001427839,0.00001033303,0.000025167],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007846604,0.00002845174,0.0004220192,0.00003602387,0.000005531065,0.0001143955,0.0000263653,0.001023857,0.9921073,0.001540789,0.0002020115,0.004414831],"study_design_scores_gemma":[0.00002094024,0.0001283132,0.0005404522,0.000004436753,0.000005849609,0.0001922785,0.00002315002,0.01467332,0.98093,0.0005957901,0.002871106,0.00001435182],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9527302,0.0004765936,0.03976694,0.0002365207,0.0001053908,0.00006468146,0.0001232007,0.001312327,0.005184104],"genre_scores_gemma":[0.9906974,0.0001099956,0.007420782,0.00003583129,0.000006051498,0.00001130654,0.00005758496,0.0000307387,0.00163042],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008528605,"threshold_uncertainty_score":0.002853096,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005145636168257194,"score_gpt":0.2476263566090828,"score_spread":0.2424807204408256,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}