{"id":"W7116745901","doi":"10.1002/sstr.202500674","title":"Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering","year":2025,"lang":"en","type":"article","venue":"Small Structures","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Ministry of Agriculture","funders":"National Key Research and Development Program of China; National Science and Technology Major Project; Chinese Universities Scientific Fund; China Agricultural University","keywords":"Inference; Key (lock); Protein engineering; Rational design; Mutagenesis; Sequence (biology); Robustness (evolution)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005151225,0.0009525679,0.0005988825,0.0004545801,0.0004036918,0.0008102147,0.0008044668,0.0008286925,0.001663282],"category_scores_gemma":[0.00117281,0.000518701,0.0006304837,0.0002486863,0.0003044185,0.0009390035,0.000552427,0.001478654,0.000661241],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00139656,"about_ca_system_score_gemma":0.001819884,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.007474968,"about_ca_topic_score_gemma":0.01159282,"domain_scores_codex":[0.9997885,0.00002721782,0.00001112325,0.00006117432,0.00008420298,0.00002788677],"domain_scores_gemma":[0.9997151,0.00009066019,0.00003707962,0.00005764839,0.00006565946,0.00003380275],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"simulation_or_modeling","study_design_scores_codex":[0.0002315783,0.0001292072,0.006126151,0.0001196005,0.0001181003,0.0001437845,0.00004628625,0.7923589,0.1311442,0.0110913,0.004029984,0.05446099],"study_design_scores_gemma":[0.000007271954,0.00002243918,0.0002141187,0.000003759424,0.000005785567,0.00002484686,0.000006206321,0.967324,0.02909146,0.002004186,0.001286375,0.000009593581],"study_design_candidate":"simulation_or_modeling","study_design_consensus":"simulation_or_modeling","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2600256,0.0005421264,0.7211415,0.0003482157,0.00006783799,0.0000545013,0.00136946,0.01102843,0.005422394],"genre_scores_gemma":[0.8174003,0.0003161899,0.1768593,0.0000989041,0.00001079964,0.0000485563,0.002208181,0.0007233943,0.002334384],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.007474968,"threshold_uncertainty_score":0.0148629,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01045060460911073,"score_gpt":0.2759199745940594,"score_spread":0.2654693699849486,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}