{"id":"W7117453256","doi":"10.1128/jcm.00872-25","title":"Systematic evaluation and optimization of TaqMan qPCR assays targeting F <i>57</i> , ISMAP <i>02</i> , and IS <i>900</i> for multiplex detection of <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i>","year":2025,"lang":"en","type":"article","venue":"Journal of Clinical Microbiology","topic":"Mycobacterium research and diagnosis","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal; Memorial University of Newfoundland; Agriculture and Agri-Food Canada","funders":"Agriculture and Agri-Food Canada; Natural Sciences and Engineering Research Council of Canada; Dairy Farmers of Canada","keywords":"TaqMan; Multiplex; Paratuberculosis; Multiplex polymerase chain reaction; Real-time polymerase chain reaction; Assay sensitivity","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0103951,0.001327161,0.001580165,0.001848267,0.0004803224,0.001582146,0.001296894,0.0009792653,0.0006523861],"category_scores_gemma":[0.009014344,0.001177664,0.001282491,0.001385126,0.001121912,0.0007470332,0.0009900621,0.0008968525,0.0007341197],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009542824,"about_ca_system_score_gemma":0.002256136,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001141894,"about_ca_topic_score_gemma":0.003093376,"domain_scores_codex":[0.9886025,0.00323101,0.00115944,0.001893837,0.004530094,0.0005832476],"domain_scores_gemma":[0.9960631,0.001102809,0.0008211001,0.0003908135,0.001510748,0.0001114702],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00011109,0.0001674486,0.003607739,0.000541726,0.00006361928,0.0000575552,0.0001825615,0.002395658,0.9685272,0.0004755473,0.0002564322,0.02361338],"study_design_scores_gemma":[0.00003029155,0.001319181,0.006031888,0.00009765426,0.0002752263,0.0002687961,0.0001584787,0.007692549,0.9745369,0.0001913479,0.009334048,0.0000635555],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6569109,0.0133325,0.3162552,0.0005171102,0.00048752,0.003291862,0.002142374,0.001233113,0.005829556],"genre_scores_gemma":[0.5718518,0.005439564,0.4114423,0.0004697009,0.0001116229,0.002223105,0.00474032,0.0002545863,0.003467057],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.0103951,"threshold_uncertainty_score":0.05497515,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03236877829540892,"score_gpt":0.3747352562908565,"score_spread":0.3423664779954476,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}