{"id":"W73745504","doi":"10.1023/a:1005675920451","title":"Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris","year":2000,"lang":"en","type":"article","venue":"Biotechnology Letters","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":40,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Universidad Nacional Autónoma de México; Institute of Genetics; Consejo Nacional de Ciencia y Tecnología","keywords":"Alcohol oxidase; Pichia pastoris; Recombinant DNA; Yeast; Saccharomyces cerevisiae; Complementary DNA; Homologous recombination; Molecular biology; Bioreactor; Biology; Pichia; Fermentation; Gene; Biochemistry; Chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00006850094,0.0001139645,0.0001359025,0.000113142,0.0000559652,0.000005277518,0.0001077545,0.0002715546,0.00003364091],"category_scores_gemma":[0.00002211375,0.0001092246,0.00004666773,0.000114526,0.0001776842,0.000003413732,0.00004839046,0.00009114832,0.000003516748],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001050124,"about_ca_system_score_gemma":0.000006192122,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001396637,"about_ca_topic_score_gemma":0.00002321841,"domain_scores_codex":[0.9992899,0.00003991466,0.0001794498,0.0002738321,0.0000490045,0.0001679187],"domain_scores_gemma":[0.9996728,0.000005035855,0.0000571061,0.0002219165,0.00001146483,0.00003167131],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005054454,0.00005824081,0.004304944,0.00001082733,0.00001680783,0.000003451444,0.00001322201,0.000003039054,0.9884271,0.0001080816,0.002944032,0.004059687],"study_design_scores_gemma":[0.0008308266,0.0006279454,0.0483241,0.00003498098,0.00001794719,0.00001897689,0.00003032233,0.00001201024,0.9413589,0.000377544,0.008104587,0.0002618787],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972678,0.0005886913,0.00006991086,0.001769221,0.00006703353,0.00009878867,0.000005636795,0.00002314832,0.0001097094],"genre_scores_gemma":[0.9983056,0.001040466,0.0001723422,0.0003439512,0.00003725758,0.00001553206,0.0000182939,0.00001200393,0.00005453605],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04706824,"threshold_uncertainty_score":0.4454049,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003965178621210016,"score_gpt":0.2080369122283131,"score_spread":0.2040717336071031,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}