{"id":"W73745504","doi":"10.1023/a:1005675920451","title":"Biosynthesis and secretion of recombinant human growth hormone in Pichia pastoris","year":2000,"lang":"en","type":"article","venue":"Biotechnology Letters","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":40,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Universidad Nacional Autónoma de México; Institute of Genetics; Consejo Nacional de Ciencia y Tecnología","keywords":"Alcohol oxidase; Pichia pastoris; Recombinant DNA; Yeast; Saccharomyces cerevisiae; Complementary DNA; Homologous recombination; Molecular biology; Bioreactor; Biology; Pichia; Fermentation; Gene; Biochemistry; Chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005554852,0.0006613409,0.0008222656,0.0003940783,0.0003140401,0.0007706291,0.0004846762,0.0003804399,0.0008913781],"category_scores_gemma":[0.0002668104,0.0003606977,0.0005026217,0.0007785892,0.0003104287,0.0003516016,0.000399432,0.0009211488,0.0008483289],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006871095,"about_ca_system_score_gemma":0.0004087108,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001213505,"about_ca_topic_score_gemma":0.001102923,"domain_scores_codex":[0.9995957,0.0000881822,0.000061629,0.00007249783,0.000105521,0.00007649911],"domain_scores_gemma":[0.9998446,0.00004191374,0.00001671725,0.00004287869,0.00001806871,0.00003583192],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001943444,0.00004362318,0.000146361,0.000111491,0.00002384238,0.000130086,0.00006346597,0.0004707426,0.9956512,0.0002822411,0.0002178999,0.002664768],"study_design_scores_gemma":[0.00004796286,0.0001738115,0.001103009,0.00001438794,0.00003596043,0.0001647013,0.00002146262,0.001142211,0.9878984,0.00008541983,0.009299697,0.00001302908],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9384415,0.005692496,0.03573301,0.0006156538,0.0004508489,0.0005443688,0.008765641,0.001122305,0.008634183],"genre_scores_gemma":[0.9268496,0.003559204,0.03313075,0.000195341,0.0001026758,0.0002489995,0.01782404,0.0002358303,0.01785355],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001213505,"threshold_uncertainty_score":0.004985332,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003965178621210016,"score_gpt":0.2080369122283131,"score_spread":0.2040717336071031,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}