{"id":"W7382634","doi":"10.1007/978-1-61779-821-4_4","title":"Protein Solubilization: Attend to the Choice of Lysis Buffer","year":2012,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"Memorial University of Newfoundland","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Lysis; Lysis buffer; Buffer (optical fiber); Solubilization; Thiourea; Chemistry; Urea; Extraction (chemistry); Chromatography; Biochemistry; Computer science; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003210356,0.00179921,0.001960693,0.001141229,0.001322129,0.001965343,0.001521908,0.001594275,0.004135246],"category_scores_gemma":[0.003208567,0.0008943386,0.0006086018,0.001237425,0.001133762,0.002340936,0.001677517,0.004029124,0.006273599],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005626775,"about_ca_system_score_gemma":0.0009513861,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005403952,"about_ca_topic_score_gemma":0.00112126,"domain_scores_codex":[0.9973382,0.000509516,0.0003376101,0.0008390307,0.0007111029,0.000264514],"domain_scores_gemma":[0.9984497,0.0005731571,0.0001765265,0.0002556408,0.0004292179,0.0001158012],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001070464,0.00007272491,0.0002382892,0.0005642483,0.00001767077,0.0001412049,0.00008847908,0.00007961722,0.9851127,0.0005390787,0.00133499,0.01170387],"study_design_scores_gemma":[0.00002559748,0.0001789731,0.001227117,0.0001172189,0.00006296684,0.001193544,0.00008433635,0.0008059912,0.9396431,0.00103231,0.05556813,0.00006066334],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.127694,0.05104138,0.7845547,0.005672489,0.003405703,0.005074555,0.002229707,0.004486653,0.01584096],"genre_scores_gemma":[0.1377763,0.05149255,0.7683087,0.003414871,0.001254244,0.005083855,0.007297177,0.001927387,0.02344482],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004135246,"threshold_uncertainty_score":0.01697814,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03384453922826196,"score_gpt":0.425665432207549,"score_spread":0.3918208929792871,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}