{"id":"W7382634","doi":"10.1007/978-1-61779-821-4_4","title":"Protein Solubilization: Attend to the Choice of Lysis Buffer","year":2012,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":29,"is_retracted":false,"has_abstract":true,"ca_institutions":"Memorial University of Newfoundland","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Lysis; Lysis buffer; Buffer (optical fiber); Solubilization; Thiourea; Chemistry; Urea; Extraction (chemistry); Chromatography; Biochemistry; Computer science; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004992777,0.00008854554,0.0001517183,0.00003797805,0.00003319063,0.000002737864,0.0002522646,0.0001169014,0.0001157819],"category_scores_gemma":[0.0003291369,0.00006689804,0.0000504286,0.000232462,0.00006142785,0.00001527802,0.0001294204,0.000127587,0.00000372625],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002487798,"about_ca_system_score_gemma":0.000009334865,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002810916,"about_ca_topic_score_gemma":0.000004628283,"domain_scores_codex":[0.9992199,0.0001560961,0.0002142927,0.0001696113,0.00003806956,0.0002020356],"domain_scores_gemma":[0.9992829,0.0001051471,0.0000794428,0.0004576052,0.00003749464,0.00003741205],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000003498851,0.00003137681,0.002417317,0.00001201669,0.000008171784,9.458175e-8,0.00004605433,0.00003291794,0.970265,0.01677625,0.0000106523,0.01039666],"study_design_scores_gemma":[0.00005930534,0.000009955837,0.00007901802,0.000008511588,0.000007746724,0.000001151953,0.00001611674,0.00007408943,0.9610291,0.008905577,0.02972974,0.00007971044],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.0458224,0.0002543307,0.9515375,0.0006084509,0.00001618877,0.0002483673,0.000006743875,0.00002448155,0.001481519],"genre_scores_gemma":[0.07610179,0.000006966609,0.9229057,0.0002064136,0.00004888604,0.0005553276,0.000009948832,0.00001361125,0.0001513085],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.03027939,"threshold_uncertainty_score":0.2728023,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03384453922826196,"score_gpt":0.425665432207549,"score_spread":0.3918208929792871,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}