{"id":"W800577","doi":"10.1042/bst20130164","title":"Programmable DNA cleavage <i>in vitro</i> by Cas9","year":2013,"lang":"en","type":"review","venue":"Biochemical Society Transactions","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"European Social Fund; Centre for Addiction and Mental Health","keywords":"Trans-activating crRNA; CRISPR; Cas9; Endonuclease; Genome editing; DNA; Biology; Computational biology; RNA; Cell biology; Genetics; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.00009315115,0.0004294321,0.0006321247,0.00002234262,0.0000701203,0.00004229347,0.0002680307,0.0008542324,0.00004229524],"category_scores_gemma":[0.000007314347,0.000409754,0.0009924188,0.0002346009,0.00009049301,0.000003371461,0.00002352735,0.0004377127,0.00003170266],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006697274,"about_ca_system_score_gemma":0.0000958982,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003822767,"about_ca_topic_score_gemma":0.000002237789,"domain_scores_codex":[0.9983015,0.00003088789,0.0004377725,0.0005976638,0.0001418728,0.0004903182],"domain_scores_gemma":[0.9993214,0.00001871057,0.00007376653,0.0003926536,0.00002488287,0.0001685593],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000006476457,0.0002531124,1.671103e-7,0.003729393,0.0003116681,0.000001226045,0.00002820441,0.000004912627,0.3467267,6.105287e-7,0.07025448,0.5786831],"study_design_scores_gemma":[0.0001767121,0.00002606106,5.749915e-8,0.0002400915,0.0001684953,0.00001552905,0.00001921102,0.00001500394,0.1403086,0.00000101761,0.8586396,0.0003895663],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.00006764346,0.9852577,0.01340672,0.00002832326,0.0001377824,0.0006717365,0.0002173569,0.00004180431,0.0001709203],"genre_scores_gemma":[0.0002540769,0.9942923,0.002175641,0.00005182582,0.0001698265,0.0004906845,0.001054424,0.00008474675,0.001426535],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.7883852,"threshold_uncertainty_score":0.9998354,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01242399919993983,"score_gpt":0.3086618710121977,"score_spread":0.2962378718122579,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}