{"id":"W891964991","doi":"10.1016/j.pep.2015.06.013","title":"Comparison of the single molecule activity distributions of recombinant and non-recombinant bovine intestinal alkaline phosphatase","year":2015,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Alkaline Phosphatase Research Studies","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Winnipeg","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Recombinant DNA; Alkaline phosphatase; Pichia pastoris; Enzyme; Chemistry; Molecular biology; Capillary electrophoresis; Biochemistry; Biology; Chromatography; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004249,0.0001219221,0.0003094787,0.00004736292,0.0001123327,0.000009922791,0.00008438936,0.00005281498,0.000008511326],"category_scores_gemma":[0.0009684136,0.0000780784,0.00003752132,0.0002030681,0.0002700469,0.000089049,0.0001732017,0.0001700821,0.000001287606],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003837846,"about_ca_system_score_gemma":0.00008740733,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001422414,"about_ca_topic_score_gemma":0.000004306781,"domain_scores_codex":[0.9989115,0.0001015708,0.0003208737,0.0002149071,0.0003028913,0.0001482706],"domain_scores_gemma":[0.9988458,0.00006544237,0.0003006009,0.0003327538,0.0003086136,0.0001467731],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008562602,0.001667697,0.01330332,0.0003789286,0.00002553973,0.000001703185,0.0003944056,0.000001001838,0.9647736,0.0005041916,0.003288014,0.01480533],"study_design_scores_gemma":[0.001356738,0.0006836038,0.02587459,0.0006283852,0.00004114676,0.000007997471,0.0002139431,0.001386022,0.9677734,0.0004238418,0.001528178,0.00008218845],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9879633,0.004786636,0.003533287,0.002230069,0.00007612478,0.001233935,0.0000760297,0.0000174172,0.00008317493],"genre_scores_gemma":[0.9978063,0.0001136188,0.001725438,0.000007085712,0.00001447099,0.00008794203,0.000100174,0.000008561755,0.0001363728],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01472314,"threshold_uncertainty_score":0.3183945,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06114805696654285,"score_gpt":0.3505832576828851,"score_spread":0.2894352007163422,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}