{"id":"W926438480","doi":"10.1016/j.pep.2015.07.005","title":"Improved biological activity of a single chain antibody fragment against human epidermal growth factor receptor 2 (HER2) expressed in the periplasm of Escherichia coli","year":2015,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"Isfahan University of Medical Sciences","keywords":"Pertuzumab; Molecular biology; Monoclonal antibody; Epidermal growth factor receptor; Antibody; Cytotoxicity; Flow cytometry; Tyrosine kinase; Biology; Epidermal growth factor; Chemistry; Periplasmic space; Escherichia coli; Receptor; Biochemistry; Trastuzumab; Cancer; Gene; Immunology; In vitro","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002500334,0.0004128837,0.0003104267,0.0002002419,0.0001090049,0.0003630603,0.0002555251,0.0002245857,0.0005407847],"category_scores_gemma":[0.0001818509,0.0001074093,0.0002536217,0.0002689607,0.0001212482,0.0001867833,0.0001604811,0.0004287345,0.0003008707],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003362606,"about_ca_system_score_gemma":0.0002539486,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001192097,"about_ca_topic_score_gemma":0.001025648,"domain_scores_codex":[0.9998161,0.00004239014,0.00002084083,0.00002747835,0.00006025968,0.00003283907],"domain_scores_gemma":[0.9999052,0.0000169587,0.00001582443,0.000009667535,0.00003324929,0.00001898742],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004920009,0.00001915968,0.00005340802,0.00002045673,0.000003130089,0.00002023119,0.000006544034,0.00002916404,0.9993619,0.00002139919,0.00001873064,0.0003967674],"study_design_scores_gemma":[0.00001015523,0.0001028427,0.001126915,0.000003471746,0.00001425217,0.0001259976,0.00001285289,0.0004356211,0.9969562,0.00001129848,0.001196411,0.00000401145],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.990914,0.001984616,0.005679542,0.0001510476,0.00004599966,0.0000158829,0.0003739172,0.00005981206,0.0007752996],"genre_scores_gemma":[0.9889453,0.00118842,0.005027211,0.00008015904,0.00001427776,0.00001588319,0.001487324,0.00003244938,0.003208885],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001192097,"threshold_uncertainty_score":0.002439737,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06509650668274142,"score_gpt":0.3267335268466056,"score_spread":0.2616370201638641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}