{"id":"W958021412","doi":"10.1007/s00253-015-6796-6","title":"Cysteine-free non-canonical C-intein for versatile protein C-terminal labeling through trans-splicing","year":2015,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"Dalhousie University","funders":"National Natural Science Foundation of China","keywords":"Intein; Protein splicing; Cysteine; Protein engineering; Chemistry; Protein tag; Biochemistry; RNA splicing; Enzyme; Fusion protein; Gene; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002936473,0.0002452468,0.0005368058,0.0001271391,0.0001230288,0.000009345316,0.0002635353,0.0008034616,0.00002243793],"category_scores_gemma":[0.00007638935,0.0001908727,0.0000852392,0.0001588244,0.000748868,0.00002884182,0.0002187971,0.0005451526,0.00003456792],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004763568,"about_ca_system_score_gemma":0.0001601744,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000929942,"about_ca_topic_score_gemma":0.00006077011,"domain_scores_codex":[0.9984697,0.00002345658,0.0003291113,0.0005217985,0.00006307737,0.0005928641],"domain_scores_gemma":[0.9992852,0.00009269947,0.00007017295,0.000350449,0.0000798058,0.0001216661],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001823505,0.00009321278,0.000082758,0.0001545988,0.000103572,0.00004502307,0.0002336322,5.131174e-7,0.9782955,0.0125615,0.001685134,0.004921053],"study_design_scores_gemma":[0.006067302,0.00235315,0.00002784468,0.0001036576,0.00008866364,0.001169533,0.0004685372,0.00008099651,0.818823,0.003510145,0.1669934,0.0003137967],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9795494,0.0007633277,0.003242454,0.01352267,0.00009707789,0.001337888,0.00008604132,0.0001215692,0.001279584],"genre_scores_gemma":[0.9780258,0.0000801269,0.0196448,0.0008002449,0.0001630901,0.0001700813,0.0001296429,0.00002607721,0.0009601238],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1653082,"threshold_uncertainty_score":0.7783564,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03082384714833213,"score_gpt":0.2926920069532126,"score_spread":0.2618681598048805,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}