{"id":"W99972145","doi":"10.1007/978-1-4939-0799-1_4","title":"Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method","year":2014,"lang":"en","type":"article","venue":"Methods in molecular biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":653,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Transformation (genetics); Yeast; Notice; PEG ratio; Saccharomyces cerevisiae; Microtiter plate; Transformation efficiency; Biology; Molecular biology; Genetics; Gene; Business; Political science; Agrobacterium","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002610612,0.0002119088,0.0002347093,0.00007826644,0.00008938647,0.00002537106,0.0004900027,0.0003368216,0.00003544051],"category_scores_gemma":[0.0004188905,0.0001557246,0.000134571,0.0002239687,0.0001816572,0.00000267723,0.000152761,0.0002759016,0.00001093702],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000166313,"about_ca_system_score_gemma":0.00004808026,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005045401,"about_ca_topic_score_gemma":0.00002520657,"domain_scores_codex":[0.9959158,0.002716051,0.0003164775,0.0004596115,0.0001296351,0.0004624534],"domain_scores_gemma":[0.9991288,0.00009129439,0.00006381749,0.0005399822,0.00007999899,0.00009614271],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004394872,0.00002687922,0.0001672338,0.00001133968,0.00003091359,7.64037e-7,0.0000734748,0.00008834375,0.9430897,0.001402033,0.0004455493,0.05461977],"study_design_scores_gemma":[0.000388659,0.0002239299,0.0000918479,0.000004066527,0.00001029476,0.00001571819,0.00004388195,0.001047265,0.8032051,0.001840931,0.1929572,0.0001711481],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.04159524,0.001000667,0.9516793,0.0006822462,0.000288842,0.0002861016,0.00003994505,0.00001108424,0.004416554],"genre_scores_gemma":[0.2782578,0.0003106554,0.7149287,0.002851492,0.000362432,0.0002979896,0.0004912828,0.0001023017,0.002397282],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.2367506,"threshold_uncertainty_score":0.6350266,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01528362098769542,"score_gpt":0.3921334445867319,"score_spread":0.3768498235990365,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}