Activation of JNK-dependent Pathway Is Required for HIV Viral Protein R-induced Apoptosis in Human Monocytic Cells
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Dossier post-publication
- Nature
- Retraction
- Motif
- Investigation by Company/Institution;Manipulation of Images;Notice - Limited or No Information;
- Date
- 1/2/2012 0:00
- Signalé par OpenAlex ?
- Oui
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Résumé
Human immunodeficiency virus (HIV) accessory protein viral protein R (Vpr) plays a key role in virus replication and induces cell cycle arrest and apoptosis in various cell types including T cells and neuronal and tumor cells following infection with Vpr-expressing HIV isolates or exposure to the extracellular Vpr protein. The C-terminal Vpr peptide encompassing amino acids 52-96 (Vpr-(52-96)) is required for exerting the apoptotic effects, whereas the N-terminal Vpr-(1-45) peptide is responsible for virus transcription. We demonstrate that Vpr-(52-96) induced apoptosis in human promonocytic THP-1 cells and primary monocytes through the mitochondrial pathway in a caspase-dependent manner. To understand the regulation of Vpr-induced apoptosis, we investigated the signaling pathways, particularly the MAPKs, and the transcription factors involved. Although both Vpr-(52-96) and Vpr-(1-45) peptides induced phosphorylation of all the three members of the MAPKs, Vpr-(52-96)-activated JNK selectively induced apoptosis in monocytic cells through the mitochondrial pathway as determined by using JNK inhibitors SP60025, dexamethasone, curcumin, and JNK-specific small interfering RNAs. Furthermore Vpr-(52-96)-induced apoptosis was mediated by inhibition of downstream antiapoptotic Bcl2 and c-IAP1 genes whose expression could be restored following pretreatment with JNK-specific inhibitors. Overall the results suggest that Vpr-(52-96)-activated JNK plays a key role in inducing apoptosis through the down-regulation of antiapoptotic Bcl2 and c-IAP1 genes.
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La notice
- Revue
- Journal of Biological Chemistry
- Thématique
- HIV Research and Treatment
- Domaine
- Immunology and Microbiology
- Établissements canadiens
- University of OttawaChildren's Hospital of Eastern Ontario
- Organismes subventionnaires
- —
- Mots-clés
- ApoptosisBiologyCell biologyKinaseSmall interfering RNAPhosphorylationSignal transductionTransfectionMolecular biologyGeneBiochemistry
- Résumé présent dans OpenAlex
- oui