Crosslinking of a Peritrophic Matrix Protein Protects Gut Epithelia from Bacterial Exotoxins
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Molecular biology of transglutaminase crosslinking in the Drosophila gut.
The study investigates insect gut biology and bacterial toxins, not research itself.
Drosophila gut barrier biology against bacterial toxins; domain immunology/microbiology.
Résumé
Transglutaminase (TG) catalyzes protein-protein crosslinking, which has important and diverse roles in vertebrates and invertebrates. Here we demonstrate that Drosophila TG crosslinks drosocrystallin, a peritrophic matrix protein, to form a stable fiber structure on the gut peritrophic matrix. RNA interference (RNAi) of the TG gene was highly lethal in flies and induced apoptosis of gut epithelial cells after oral infection with Pseudomonas entomophila. Moreover, AprA, a metalloprotease secreted by P. entomophila, digested non-crosslinked drosocrystallin fibers, but not drosocrystallin fibers crosslinked by TG. In vitro experiments using recombinant drosocrystallin and monalysin proteins demonstrated that monalysin, a pore-forming exotoxin of P. entomophila, was adsorbed on the crosslinked drosocrystallin fibers in the presence of P. entomophila culture supernatant. In addition, gut-specific TG-RNAi flies had a shorter lifespan than control flies after ingesting P. entomophila, whereas the lifespan after ingesting AprA-knockout P. entomophila was at control levels. We conclude that drosocrystallin fibers crosslinked by TG, but not non-crosslinked drosocrystallin fibers, form an important physical barrier against exotoxins of invading pathogenic microbes.
Conservé avec la notice de tri, où il sert de preuve aux étiquettes ci-dessus.
La notice
- Revue
- PLoS Pathogens
- Thématique
- Invertebrate Immune Response Mechanisms
- Domaine
- Immunology and Microbiology
- Établissements canadiens
- —
- Organismes subventionnaires
- Institute of GeneticsUehara Memorial Foundation
- Mots-clés
- BiologyMicrobiologyIn vitroRecombinant DNARNA interferenceCell biologyBiochemistryRNAGene
- Résumé présent dans OpenAlex
- oui