Comparison of a Manual and an Automated Method to Estimate the Number of Uterine Eggs in Anisakid Nematodes: To Coulter or Not to Coulter. Is That the Question?
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Le tri à trois modèles
les 1 000 travaux triés →1 modèle sur 3 a qualifié ce travail de métarecherche. Ce travail est contesté : il se situe à la frontière empirique du domaine, et son statut dépend du modèle interrogé. C'est l'un des 51 travaux du dossier des désaccords.
Comparison of two laboratory techniques for counting nematode eggs; this is assay/measurement validation within parasitology, the polysemy trap the rubric flags, not a study of research practice.
The primary object is a comparison of two research measurement methods and their precision, although it is narrowly domain-specific.
Compares laboratory egg-counting techniques in parasitology; domain measurement methods, not research practice as object.
Résumé
Studies reporting numbers of eggs in vagina and utero in nematodes often give little information of the technique used for the estimations. This situation hampers comparison among studies, because, so far, differences in estimations provided by different techniques have not been assessed. This note examines whether a manual method based on visual counts in aliquots and an automated method using a Coulter counter yield equivalent estimations of egg numbers in vagina and utero of 3 anisakid nematode species (Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum). The number of eggs from 50 females per nematode species was estimated using both techniques. The automated and manual methods yielded similar egg counts (correlation coefficients >0.9 in the 3 species), but the methods were not always statistically equivalent. The automated method was more precise and seemed less dependent on egg density, whereas the manual method was less time-consuming (contrary to previous perceptions) and less expensive. Despite the higher precision of automated counts, the manual technique seemed to produce similar estimates; thus, it may be particularly useful in developing countries where nematode parasitism is prevalent in humans and domestic animals, but scientific resources are limited.
Conservé avec la notice de tri, où il sert de preuve aux étiquettes ci-dessus.
La notice
- Revue
- Journal of Parasitology
- Thématique
- Parasite Biology and Host Interactions
- Domaine
- Environmental Science
- Établissements canadiens
- Environment and Climate Change Canada
- Organismes subventionnaires
- —
- Mots-clés
- BiologyNematodeCoulter counterAnisakis simplexHelminthsZoologyEcologyLarva
- Résumé présent dans OpenAlex
- oui