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<i>Retracted</i>: miR‐942 promotes tumor migration, invasion, and angiogenesis by regulating EMT via BARX2 in non‐small‐cell lung cancer

2019· article· en· 41 citations· W2953568798 sur OpenAlex· 10.1002/jcp.28928

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Dossier post-publication

Nature
Retraction
Motif
Concerns/Issues about Data;Concerns/Issues about Results and/or Conclusions;Investigation by Journal/Publisher;Objections by Third Party;Unreliable Results and/or Conclusions;
Date
3/7/2022 0:00
Signalé par OpenAlex ?
Oui

Source : Retraction Watch, jointe par DOI. OpenAlex consigne la rétractation dans is_retracted, un booléen sur un espace d'états à au moins quatre valeurs ; il ne peut donc exprimer ni une expression de préoccupation, ni une correction, ni un rétablissement, et les rapporte comme false, ce qui se lit comme « rien à signaler ».

Résumé

Epithelial-mesenchymal transition (EMT) has an important function in cancer. Recently, microRNAs have been reported to be involved in EMT by regulating target genes. miR-942 is considered a novel oncogene in esophageal squamous cell carcinoma. However, its role in non-small-cell lung cancer (NSCLC) has not been investigated. In this study, the expression of miR-942 in NSCLC patients tumor and paired adjacent tissues were assessed by quantitative real-time polymerase chain reaction and in situ hybridization. Transwell, wound healing, tube formation, and tail vein xenograft assays were conducted to assess miR-942's function in NSCLC. Potential miR-942 targets were confirmed using dual-luciferase reporter assays, immunohistochemistry, immunoblot, and rescue experiments. The results showed miR-942 is relatively highly expressed in human NSCLC tissues and cells. In vitro assays demonstrated that overexpression of miR-942 promoted cell migration, invasion, and angiogenesis. Tail vein xenograft assays suggested that miR-942 contributed to NSCLC metastasis in vivo. Three bioinformatics software was searched, and BARX2 was predicted as a downstream target of miR-942. Direct interaction between them was validated by dual-luciferase assays. Rescue experiments further confirmed that BARX2 overexpression could reverse functional changes caused by miR-942. Moreover, miR-942 increased EMT-associated proteins N-cadherin and vimentin by inhibiting BARX2, while E-cadherin expression is reduced. In summary, this study reveals that miR-942 induces EMT-related metastasis by directly targeting BARX2, which may provide a potential therapeutic strategy for NSCLC.

Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.

La notice

Revue
Journal of Cellular Physiology
Thématique
Cancer-related molecular mechanisms research
Domaine
Biochemistry, Genetics and Molecular Biology
Établissements canadiens
CAE (Canada)
Organismes subventionnaires
Natural Science Foundation of Jiangsu ProvinceNational Natural Science Foundation of China
Mots-clés
AngiogenesisCancer researchCancerCell migrationCellLungLung cancerBiologyMedicinePathologyInternal medicineGenetics
Résumé présent dans OpenAlex
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