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An inexpensive, automation‐friendly protocol for recovering high‐quality DNA

2006· article· en· 1,476 citations· W1605875024 on OpenAlex· 10.1111/j.1471-8286.2006.01428.x

Why is this work in the frame?

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

Canadian affiliationAn author listed a Canadian institution. This is the only route the usual frame has.
Canadian funderA Canadian agency funded it. The work may carry no Canadian affiliation at all.

Machine scores (provisional)

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Opus teacher head0.022
GPT teacher head0.342
Teacher spread
0.320 · how far apart the two teachers sit on this one work
Validation status
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Abstract

Abstract Although commercial kits are available for automated DNA extraction, ‘artisanal’ protocols are not. In this study, we present a silica‐based method that is sensitive, inexpensive and compliant with automation. The effectiveness of this protocol has now been tested on more than 5000 animal specimens with highly positive results.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

The record

Venue
Molecular Ecology Notes
Topic
Identification and Quantification in Food
Field
Biochemistry, Genetics and Molecular Biology
Canadian institutions
University of Guelph
Funders
Natural Sciences and Engineering Research Council of CanadaCanada Research ChairsOntario Genomics InstituteGenome CanadaGordon and Betty Moore Foundation
Keywords
AutomationProtocol (science)DNA extractionComputer scienceEmbedded systemBiologyEngineeringMedicinePolymerase chain reactionPathologyGenetics
Has abstract in OpenAlex
yes