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Sperm Preparation Methods

2000· article· en· W1961154935 on OpenAlexaff
David Mortimer

Bibliographic record

VenueJournal of Andrology · 2000
Typearticle
Languageen
FieldMedicine
TopicSperm and Testicular Function
Canadian institutionsCReATe Fertility Centre
Fundersnot available
KeywordsSpermAndrologyChemistryMedicine

Abstract

fetched live from OpenAlex

The spermatozoa of all placental (eutherian) mammals, including humans, are in a protective, nonlabile state at ejaculation and are incapable of fertilization even if they are placed in direct contact with an oocyte.Consequently, they must undergo a subsequent period of final maturation during which they acquire the capacity to interact with the oocyte-cumulus complex and achieve fertilization.This process, which was discovered independently by Austin and Chang in 1951, was termed capacitation, and spermatozoa in the ejaculate are prevented from undergoing capacitation by one or more decapacitation factors that are present in the seminal plasma (Yanagimachi, 1994).Capacitation of eutherian spermatozoa is essential for fertilization not only in vivo but also in vitro, and underlies the manipulation of spermatozoa for clinical in vitro fertilization (IVF).Not only does seminal plasma contain one or more decapacitation factors that prevent spontaneous capacitation of spermatozoa upon ejaculation, but it also contains one or more factors to which prolonged exposure has adverse effects on sperm function, including the ability to penetrate cervical mucus (Kremer, 1968), undergo the acrosome reaction in vitro, and the fertilization process in general (Rogers et al, 1983;Mortimer and Mortimer, 1992;Mortimer et al, 1998).Consequently, in order for eutherian spermatozoa to have the capacity to fertilize an oocyte, they must be separated from the seminal plasma, and hence, the separation of human spermatozoa from seminal plasma is an essential prerequisite for them to be able to achieve capacitation and express their intrinsic fertilizing ability.In assisted reproductive technology (ART) laboratories, this need is manifested in the process commonly referred to as ''sperm washing,'' in which spermatozoa are somehow removed from the seminal plasma and resuspended in culture medium.Prolonged exposure (Ͼ30 minutes) to seminal plasma after ejaculation can permanently diminish the fertilizing capacity of human spermatozoa in vitro (Rogers et al, 1983), and contamination of prepared sperm populations with only traces of seminal plasma can diminish, or even

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.003
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.158
Threshold uncertainty score0.530

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0030.003
Meta-epidemiology (narrow)0.0030.001
Meta-epidemiology (broad)0.0030.002
Bibliometrics0.0050.003
Science and technology studies0.0030.001
Scholarly communication0.0020.001
Open science0.0040.002
Research integrity0.0020.003
Insufficient payload (model declined to judge)0.1580.111

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.367
Teacher spread0.348 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations179
Published2000
Admission routes1
Has abstractyes

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Same venueJournal of AndrologySame topicSperm and Testicular FunctionFrench-language works237,207