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Record W1963654627 · doi:10.1002/cyto.a.20623

MIFlowCyt: The minimum information about a flow cytometry experiment

2008· article· en· W1963654627 on OpenAlexafffund
Jamie A. Lee, Josef Špidlen, Keith Boyce, Jennifer Cai, Nicholas D. Crosbie, Mark E. Dalphin, Jeff Furlong, Maura Gasparetto, Michael Goldberg, Elizabeth M. Goralczyk, Bill Hyun, Kirstin Jansen, Tobias R. Kollmann, Megan Kong, Robert C. Leif, Shannon K. McWeeney, Thomas D. Moloshok, Wayne Moore, Garry P. Nolan, John P. Nolan, Janko Nikolich‐Žugich, David B. Parrish, Barclay Purcell, Qian Yu, Biruntha Selvaraj, Clayton A. Smith, Olga Tchuvatkina, Anne M. Wertheimer, Peter Wilkinson, Christopher Wilson, James C. S. Wood, Robert Zigon, Richard H. Scheuermann, Ryan R. Brinkman

Bibliographic record

VenueCytometry Part A · 2008
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsUniversité de MontréalChild and Family Research InstituteUniversity of British ColumbiaBC Cancer Agency
FundersNational Institute of Biomedical Imaging and BioengineeringNational Institute of Allergy and Infectious DiseasesNational Institute on AgingNational Institutes of HealthMichael Smith Health Research BCInternational Society for Infectious Diseases
KeywordsComputer scienceSoftwareCytometryReuseData sciencePublishingFlow cytometryBiology

Abstract

fetched live from OpenAlex

A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoptionof MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.051
metaresearch head score (Gemma)0.089
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesMetaresearch
Consensus categoriesnone
DomainCandidate signal: Reporting · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: Not applicable
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.949
Threshold uncertainty score0.268

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0510.089
Meta-epidemiology (narrow)0.0020.003
Meta-epidemiology (broad)0.0030.002
Bibliometrics0.0090.005
Science and technology studies0.0050.003
Scholarly communication0.0080.007
Open science0.0080.007
Research integrity0.0080.006
Insufficient payload (model declined to judge)0.0370.038

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.234
Teacher spread0.216 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

Study designNot applicable
DomainReporting
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations514
Published2008
Admission routes2
Has abstractyes

Explore more

Same venueCytometry Part ASame topicSingle-cell and spatial transcriptomicsFrench-language works237,207