RETRACTED: Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Post-publication record
- Nature
- Retraction
- Reason
- Falsification/Fabrication of Data;Falsification/Fabrication of Image;Investigation by Company/Institution;Investigation by ORI;Misconduct - Official Investigation(s) and/or Finding(s);Misconduct by Author;
- Date
- 3/12/2018 0:00
- Flagged by OpenAlex?
- Yes
Source: Retraction Watch, joined by DOI. OpenAlex records retraction as is_retracted, a boolean over a state space with at least four values, so it cannot express an expression of concern, a correction or a reinstatement — it reports them as false, which reads as “fine”.
Abstract
Actin filaments and integrin-based focal adhesions (FAs) form integrated systems that mediate dynamic cell interactions with their environment or other cells during migration, the immune response, and tissue morphogenesis. How adhesion-associated actin structures obtain their functional specificity is unclear. Here we show that the formin-family actin nucleator, inverted formin 2 (INF2), localizes specifically to FAs and dorsal stress fibers (SFs) in fibroblasts. High-resolution fluorescence microscopy and manipulation of INF2 levels in cells indicate that INF2 plays a critical role at the SF-FA junction by promoting actin polymerization via free barbed end generation and centripetal elongation of an FA-associated actin bundle to form dorsal SF. INF2 assembles into FAs during maturation rather than during their initial generation, and once there, acts to promote rapid FA elongation and maturation into tensin-containing fibrillar FAs in the cell center. We show that INF2 is required for fibroblasts to organize fibronectin into matrix fibers and ultimately 3D matrices. Collectively our results indicate an important role for the formin INF2 in specifying the function of fibrillar FAs through its ability to generate dorsal SFs. Thus, dorsal SFs and fibrillar FAs form a specific class of integrated adhesion-associated actin structure in fibroblasts that mediates generation and remodeling of ECM.
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The record
- Venue
- Proceedings of the National Academy of Sciences
- Topic
- Cellular Mechanics and Interactions
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of Toronto
- Funders
- National Institutes of Health
- Keywords
- ForminsExtracellular matrixAdhesionFocal adhesionDorsumCell biologyStress fiberChemistryFiberMatrix (chemical analysis)ActinBiophysicsAnatomyBiologyBiochemistryActin cytoskeletonCytoskeletonSignal transductionCellChromatography
- Has abstract in OpenAlex
- yes