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Record W2035596374 · doi:10.1089/cmb.2009.0047

Maximum Likelihood Genome Assembly

2009· article· en· W2035596374 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueJournal of Computational Biology · 2009
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsUniversity of Toronto
FundersNational Institute of General Medical SciencesNatural Sciences and Engineering Research Council of CanadaNational Institutes of Health
KeywordsGenomeDe Bruijn graphDe Bruijn sequenceContigHybrid genome assemblySequence assemblyComputational biologyBiologyGeneticsComputer scienceAlgorithmMathematicsCombinatoricsGene

Abstract

fetched live from OpenAlex

Whole genome shotgun assembly is the process of taking many short sequenced segments (reads) and reconstructing the genome from which they originated. We demonstrate how the technique of bidirected network flow can be used to explicitly model the double-stranded nature of DNA for genome assembly. By combining an algorithm for the Chinese Postman Problem on bidirected graphs with the construction of a bidirected de Bruijn graph, we are able to find the shortest double-stranded DNA sequence that contains a given set of k-long DNA molecules. This is the first exact polynomial time algorithm for the assembly of a double-stranded genome. Furthermore, we propose a maximum likelihood framework for assembling the genome that is the most likely source of the reads, in lieu of the standard maximum parsimony approach (which finds the shortest genome subject to some constraints). In this setting, we give a bidirected network flow-based algorithm that, by taking advantage of high coverage, accurately estimates the copy counts of repeats in a genome. Our second algorithm combines these predicted copy counts with matepair data in order to assemble the reads into contigs. We run our algorithms on simulated read data from Escherichia coli and predict copy counts with extremely high accuracy, while assembling long contigs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.948
Threshold uncertainty score0.324

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.257
Teacher spread0.248 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it