A DNA barcode for land plants
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Abstract
DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF-atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK-psbI spacer, and trnH-psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
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The record
- Venue
- Proceedings of the National Academy of Sciences
- Topic
- Genomics and Phylogenetic Studies
- Field
- Biochemistry, Genetics and Molecular Biology
- Canadian institutions
- University of TorontoUniversity of British ColumbiaUniversity of Guelph
- Funders
- National Institutes of HealthU.S. National Library of MedicineAlfred P. Sloan FoundationNational Research FoundationGenome CanadaGordon and Betty Moore Foundation
- Keywords
- BarcodeDNA barcodingrpoBBiologyGeneLocus (genetics)DNA sequencingGeneticsComputational biologyDNAPolymerase chain reactionEvolutionary biologyComputer science16S ribosomal RNA
- Has abstract in OpenAlex
- yes