Potential of the Mycoparasite, <i>Verticillium lecanii</i>, to Protect Citrus Fruit Against <i>Penicillium digitatum</i>, the Causal Agent of Green Mold: A Comparison with the Effect of Chitosan
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
ABSTRACT The potential of the mycoparasite, Verticillium lecanii, at protecting citrus fruits against green mold was explored at the cellular level. Treatmentthe fruit with V. lecanii or chitosan prior to inoculation with the causal agent of green mold, Penicillium digitatum, markedly reduced disease development compared with that of nontreated control citrus fruits in which symptoms were visible by 3 days after inoculation with the pathogen. Scanning electron microscope investigations of citrus samples, collected 5 days after inoculation with the pathogen, revealed striking differences in the extent of cell surface colonization between treated and nontreated fruits. Pathogen hyphae, which sporulated abundantly at the surface of control fruits, were collapsed and severely damaged in V. lecanii and chitosan-treated fruits. Histological observations of citrus samples confirmed that restriction of pathogen colonization at the cell surface correlated with a pronounced disorganization of the pathogen hyphae. In addition, host cell changes, mainly characterized by the deposition of a new material in the exocarp cells and the thickening of cell walls, were observed. Ultrastructural investigations of citrus samples revealed that the pathogen multiplied abundantly through much of the mesocarp and exocarp tissues in V. lecanii-free citrus fruits, whereas in V. lecanii-treated citrus, pathogen growth was restricted. Penicillium hyphae that penetrated the mesocarp tissue were markedly altered. Labeling with the wheat germ agglutinin/ovomucoid-gold complex for the localization of chitin resulted in an irregular labeling of Penicillium cell walls, even at a time when in an irregular labeling of Penicillium cell walls, even at a time when these were markedly altered. Cytochemical investigations revealed that callose and lignin-like compounds accumulated at sites of pathogen colonization in the exocarp tissue. Evidence is provided in this study that V. lecanii as well as chitosan are equally capable of inducing a striking response in P. digitatum-infected citrus fruits. The marked differences observed in the rate and extent of colonization as well as in pathogen cell viability between control and treated citrus fruits demonstrate that both treatments have the ability to induce the transcriptional activation of defense genes leading to the accumulation of structural and biochemical compounds at strategic sites.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it