M-DNA: pH Stability, Nuclease Resistance and Signal Transmission
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Abstract In the presence of divalent metal ions (Zn(2)+, Co(2)+, and Ni(2)+) and at pHs above 8, duplex DNA forms a complex called M-DNA. M-DNA can be converted back to B-DNA by addition of EDTA or lowering the pH. The stability of M-DNA depends on the metal ion and/or the sequence of DNA. For calf thymus DNA the order of stability with decreasing pH is: Ni(2+)> Co(2+)>Zn(2++). The interconversion with B-DNA shows hysteresis; once formed Ni-M- DNA remains stable for more than one hour at pH 7, but conversion of B-DNA to M-DNA is slow at pHs below 8. Among synthetic sequences, poly[d(AT)] does not form M-DNA whereas the phosphorothioate analogues form only at pH 9.0. In contrast, the Ni-M-DNA form of poly[d(GC)] is stable even at pH 6.5. Ni-M-DNA is resistant to cleavage by DNase I whereas B-DNA is digested rapidly under identical conditions. The Co(2)+ and Ni(2+) forms of M-DNA were paramagnetic with increased mass susceptibilities (χ) compared to other metal complexes. Signal transmission in M-DNA was tested by constructing duplexes of 54 base pairs with fluorescein (donor) at one end and rhodamine (acceptor) at the other. Quenching of fluorescein fluorescence was observed for the Zn(2+) form of M-DNA only when the DNA was labeled with both donor and acceptor. Therefore, the pathway of quenching maybe via electron transfer. Taken together, these results suggest that M-DNA is a distinct conformation with tightly bound metal ions, and certain forms may be stable under physiological conditions. Furthermore, M-DNA may be used as a molecular wire for signal transmission over long distances.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it