Construction and analysis of pathogenicity island deletion mutants of <i>Erwinia amylovora</i>
Why this work is in the frame
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Bibliographic record
Abstract
An easy gene-knockout technique, PCR-based one-step inactivation of chromosomal genes, is widely used in Escherichia coli and related enterobacteria to construct mutants. In this study, we adapted this technique to construct genomic island and large operon deletion mutants of Erwinia amylovora, including the 33.4 kb hrp-type III secretion (T3SS) pathogenicity island (PAI1) and the 15.8 kb amylovoran biosynthesis (AMS) operon. Deletion of 2 novel T3SS pathogenicity islands (PAI2 and PAI3) and an operon encoding a type II secretion system (T2SS) demonstrated that these determinants are not involved in virulence in plants. Co-inoculation experiments demonstrated that the hrp-T3SS and AMS deletion mutants could complement each other. These results further confirmed that the one-step inactivation technique can be used to generate large deletions in E. amylovora.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it