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Search for folding nuclei in native protein structures

2005· article· en· W2108926360 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueComputer applications in the biosciences · 2005
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicProtein Structure and Dynamics
Canadian institutionsUniversity of British Columbia
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsExecutableComputer scienceProtein foldingFolding (DSP implementation)Simple (philosophy)AlgorithmGraphTheoretical computer scienceTopology (electrical circuits)PhysicsMathematicsCombinatoricsEngineering

Abstract

fetched live from OpenAlex

UNLABELLED: The problem of finding folding nuclei (a set of native contacts that play an important role in folding) along with identifying folding pathways (a time-ordered sequence of folding events) of proteins is one of the most important problems in protein chemistry. Here we propose a novel and simple approach to address this problem as follows: given the topology of the native state, identify native contacts that form folding nuclei based on a graph-theoretical approach that considers effective contact order (effective loop closure) as its objective function. MOTIVATION: A number of computational methods for the prediction of folding nuclei already exists in the literature, but most of them rely on restrictive assumptions about the nature of nuclei or the process of folding. Our motivation is to develop a simple, efficient and robust algorithm to find an ensemble of pathways with the lowest effective contact order and to identify contacts that are crucial for folding. RESULTS: Our approach is different from the previously used methods in that it uses efficient graph algorithms and does not formulate restrictive assumptions about folding nuclei. Our predictions provide more details concerning the protein folding pathway than most other methods in the literature. We demonstrate the success of our approach by predicting folding nuclei for a dataset of proteins for which experimental kinetic data is available. We show that our method compares favourably with other methods in the literature and that its results agree with experimental results. AVAILABILITY: The executable for the proposed algorithm is available at http://www.cs.ubc.ca/~/foldingnuclei.html

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.270
Threshold uncertainty score0.205

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.297
Teacher spread0.283 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it