MétaCan
Menu
Back to cohort

Reflected‐Light Optical Microscopy

2012· other· en· W2118947349 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueCharacterization of Materials · 2012
Typeother
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicAdvanced Fluorescence Microscopy Techniques
Canadian institutionsMcGill University
FundersCanada Research Chairs
KeywordsMicroscopyLight sheet fluorescence microscopyBright-field microscopyOpticsOpacityPolarized light microscopyDifferential interference contrast microscopyOptical microscopeInstrumentation (computer programming)Phase contrast microscopyMaterials scienceInterference microscopyComputer scienceScanning confocal electron microscopyPhysics

Abstract

fetched live from OpenAlex

Abstract Imaging of opaque specimens is performed using reflected rather than transmitted light. Illumination is supplied from above using orientations ranging from on‐axis (brightfield imaging) to highly oblique (oblique‐light microscopy; darkfield). The illuminating light might also be polarized (polarized light microscopy; differential interference contrast) or phase‐advanced or ‐retarded (phase contrast microscopy). Each of these techniques leads to generation of contrast from different features of a specimen, and several techniques can be used to complement each other and provide information about specimen composition, feature size, height, and other properties. Reflected‐light illumination is also the most common and most sensitive way to perform fluorescence microscopy on both transparent and opaque specimens. This article covers the principles behind the major techniques of reflected‐light optical microscopy and gives examples of materials applications, both traditional and emerging. The set‐up of the instrumentation required for each technique is given in detail, and Section “Practical Aspects of the Method” discusses the precise instrumentation and accessories needed to implement each technique. We also provide a guide to the most common imaging artifacts and pitfalls in reflected‐light microscopy.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow), Insufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Other · Consensus signal: none
Teacher disagreement score0.625
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0010.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.282
Teacher spread0.274 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it