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Record W2147698799 · doi:10.1096/fj.06-075820

Selenoprotein T is a PACAP‐regulated gene involved in intracellular Ca <sup>2+</sup> mobilization and neuroendocrine secretion

2008· article· en· W2147698799 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueThe FASEB Journal · 2008
Typearticle
Languageen
FieldNursing
TopicSelenium in Biological Systems
Canadian institutionsInstitut National de la Recherche ScientifiqueMontreal Heart InstituteUniversité du Québec à Montréal
Fundersnot available
KeywordsIntracellularSecretionSelenoprotein PCell biologyGeneChemistryMobilizationEndocrinologyBiologyMolecular biologyBiochemistryEnzymeGlutathionePolitical science

Abstract

fetched live from OpenAlex

Selenoproteins contain the essential trace element selenium, the deficiency of which is associated with cancer or accelerated aging. Although selenoproteins are thought to be instrumental for the effects of selenium, the biological function of many of these proteins remains unknown. Here, we studied the role of selenoprotein T (SelT), a selenocysteine (Sec) ‐containing protein with no known function, which we have identified as a novel target gene of the neuropeptide pituitary adenylate cyclase‐activating polypeptide (PACAP) during PC12 cell differentiation. SelT was found to be ubiquitously expressed throughout embryonic development and in adulthood in rat. Immunocytochemical analysis revealed that SelT is mainly localized to the endoplasmic reticulum through a hydrophobic domain. PACAP and cAMP induced a rapid and long‐lasting increase in SelT gene expression in PC12 cells, in a Ca 2+ ‐dependent manner. These results suggested a possible role of SelT in PACAP signaling during PC12 cell differentiation. Indeed, overexpression of SelT in PC12 cells provoked an increase in the concentration of intracellular Ca 2+ ([Ca 2+ ] i ) that was dependent on the Sec residue. Conversely, SelT gene knockdown inhibited the PACAP‐induced increase in [Ca 2+ ] i and reduced hormone secretion. These findings demonstrate the implication of a selenoprotein in the regulation of Ca 2+ homeostasis and neuroendocrine secretion in response to a cAMP‐stimulating trophic factor.— Grumolato, L., Ghzili, H., Montero‐Hadjadje, M., Gasman, S., Lesage, J., Tanguy, Y., Galas, L., Ait‐Ali, D., Leprince, J., Guerineau, N. C., Elkahloun, A. G., Fournier, A., Vieau, D., Vaudry, H., Anouar, Y. Selenoprotein T is a PACAP‐regulated gene involved in intracellular Ca2+ mobilization and neuroendocrine secretion. FASEB J. 22, 1756–1768 (2008)

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.207
Threshold uncertainty score0.597

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.228
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it