Identification of Larvae of Exotic <I>Tipula paludosa</I> (Diptera: Tipulidae) and <I>T. oleracea</I> in North America Using Mitochondrial <I>cytB</I> Sequences
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Bibliographic record
Abstract
Two exotic crane fly species, Tipula (Tipula) paludosa Meigen (Diptera: Tipulidae) and Tipula (Tipula) oleracea L. have spread considerably in North America beyond original areas of detection in eastern and western Canada. These species are endemic in Europe, and pests in pastures and cereals. The two species differ in life cycles and periods when they feed, warranting species-specific control. Identification presents a challenge because the larvae are not easily distinguishable from each other, and resemble native nonpestiferous species present in sympatry. We collected crane flies from urban landscapes and agricultural fields in Oregon in the western United States. Using polymerase chain reaction (PCR), we sequenced a portion of the mitochondrial cytB gene in 55 individuals (eight adults and 47 larvae) from 29 sites. We observed 7% divergence between exotic and native species. Phylogenetic analysis, using Nephrotoma ferruginea F. as an outgroup, resolved four well-supported monophyletic groups: the exotics, T. (Tipula) oleracea and T. (Tipula) paludosa, and two natives, Tipula (Serratipula) tristis Doane and Tipula (Triplicitipula) sp. Nucleotide divergence between T. oleracea and T. paludosa was P = 0.071, whereas within species divergence was very low (P = 0.0018 and P = 0.0022, respectively). The study indicated that mitochondrial cytB sequences provided an accurate, rapid, and economic technique for separation of T. oleracea and T. paludosa from each other and from native species, and insights on their habitats. The technique will facilitate early pest management decisions, and studies on host plants and geographic distribution, as the two exotic species extend their ranges across North America.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.002 | 0.002 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.001 | 0.003 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.002 | 0.001 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it