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Record W2351559594 · doi:10.1136/jclinpath-2015-203502

Laboratory assessment of vitamin B<sub>12</sub> status

2016· article· en· W2351559594 on OpenAlexaff
Dominic J. Harrington

Bibliographic record

VenueJournal of Clinical Pathology · 2016
Typearticle
Languageen
FieldMedicine
TopicFolate and B Vitamins Research
Canadian institutionsSt. Thomas Hospital
FundersUniversity Hospitals Birmingham NHS Foundation Trust
KeywordsVitamin B12Methylmalonic acidHomocysteineMethionine synthaseAdenosylcobalaminMethylcobalaminMethionineInternal medicineBiochemistryMedicineEndocrinologyPernicious anaemiaCyanocobalaminReference rangeChemistryCobalaminAmino acid

Abstract

fetched live from OpenAlex

The detection and correction of vitamin B 12 (B 12 ) deficiency prevents megaloblastic anaemia and potentially irreversible neuropathy and neuropsychiatric changes. B 12 status is commonly estimated using the abundance of the vitamin in serum, with ∼148 pmol/L (200 ng/L) typically set as the threshold for diagnosing deficiency. Serum B 12 assays measure the sum of haptocorrin-bound and transcobalamin-bound (known as holotranscobalamin) B 12 . It is only holotranscobalamin that is taken up by cells to meet metabolic demand. Although receiver operator characteristic curves show holotranscobalamin measurement to be a moderately more reliable marker of B 12 status than serum B 12 , both assays have an indeterminate range. Biochemical evidence of metabolic abnormalities consistent with B 12 insufficiency is frequently detected despite an apparently sufficient abundance of the vitamin. Laboratory B 12 status markers that reflect cellular utilisation rather than abundance are available. Two forms of B 12 act as coenzymes for two different reactions. Methionine synthase requires methylcobalamin for the remethylation of methionine from homocysteine. A homocysteine concentration &gt;20 µmol/L may suggest B 12 deficiency in folate-replete patients. In the second B 12 -dependent reaction, methylmalonyl-CoA mutase uses adenosylcobalamin to convert methylmalonyl-CoA to succinyl-CoA. In B 12 deficiency excess methylmalonyl-CoA is hydrolysed to methylmalonic acid. A serum concentration &gt;280 nmol/L may suggest suboptimal status in young patients with normal renal function. No single laboratory marker is suitable for the assessment of B 12 status in all patients. Sequential assay selection algorithms or the combination of multiple markers into a single diagnostic indicator are both approaches that can be used to mitigate inherent limitations of each marker when used independently.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.006
Threshold uncertainty score0.020

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0030.001
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0060.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.067
GPT teacher head0.463
Teacher spread0.396 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations132
Published2016
Admission routes1
Has abstractyes

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