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Record W2411083416 · doi:10.1385/1-59259-284-8:377

Crosslinking of Proteins to mtDNA

2003· article· en· W2411083416 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenueMitochondrial DNA · 2003
Typearticle
Languageen
FieldMaterials Science
TopicDiatoms and Algae Research
Canadian institutionsXenon Pharmaceuticals (Canada)
FundersNational Institute of General Medical SciencesNational Institutes of HealthWelch Foundation
KeywordsNucleoidMitochondrial DNABiologyBiochemistryDNAMitochondrionPhysarum polycephalumInner mitochondrial membraneCell biologyEscherichia coliGene

Abstract

fetched live from OpenAlex

Mitochondrial DNA (mtDNA) nucleoids have been isolated from several organisms, including rat (liver) (), Physarum polycephalum (), Saccharomy- ces cerevisiae () and Pichia jadinii (). Most methods for nucleoid isolation have utilized detergent extraction and sucrose gradient centrifugation to separate bulk mitochondrial protein from mtDNA-associated proteins (e.g., ref. 5). The disadvantages of these methods include contamination by non-nucleoid proteins and the possibility of losing those nucleoid proteins that are not tightly bound to the complex under the conditions of the fractionation. To circumvent these problems, we have developed an in organello formaldehyde crosslinking method for isolating mtDNA nucleoids that stabilizes protein content and reduces the potential for contamination by irrelevant mitochondrial (and cellular) proteins. Additionally, it has long been suggested that mtDNA is associated with the inner membrane. To begin exploring that notion, we have used ultraviolet (UV) crosslinking as a means of detecting the binding of proteins from membrane-enriched fractions to specific sequences of mtDNA.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesInsufficient payload (model declined to judge)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.038
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.284
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it