An environment-dependent transcriptional network specifies human microglia identity
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Abstract
Microglia play essential roles in central nervous system (CNS) homeostasis and influence diverse aspects of neuronal function. However, the transcriptional mechanisms that specify human microglia phenotypes are largely unknown. We examined the transcriptomes and epigenetic landscapes of human microglia isolated from surgically resected brain tissue ex vivo and after transition to an in vitro environment. Transfer to a tissue culture environment resulted in rapid and extensive down-regulation of microglia-specific genes that were induced in primitive mouse macrophages after migration into the fetal brain. Substantial subsets of these genes exhibited altered expression in neurodegenerative and behavioral diseases and were associated with noncoding risk variants. These findings reveal an environment-dependent transcriptional network specifying microglia-specific programs of gene expression and facilitate efforts to understand the roles of microglia in human brain diseases.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Science
- Topic
- Neuroinflammation and Neurodegeneration Mechanisms
- Field
- Neuroscience
- Canadian institutions
- —
- Funders
- National Institute of Diabetes and Digestive and Kidney DiseasesNational Institute of Neurological Disorders and StrokeNational Institute of General Medical SciencesUniversity of California, San DiegoNational Cancer InstituteNational Institutes of HealthCanadian Institutes of Health ResearchMultiple Sclerosis SocietyMultiple Sclerosis Society of Canada
- Keywords
- MicrogliaEpigeneticsBiologyGeneImmune systemEx vivoGene expressionFunction (biology)PhenotypeIn vitroNeuroscienceHuman brainCell biologyImmunologyGeneticsInflammation
- Has abstract in OpenAlex
- yes