Role of the TSLP–DC–OX40L pathway in asthma pathogenesis and airway inflammation in mice
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
Post-publication record
- Nature
- Retraction
- Reason
- Author Unresponsive;Duplication of/in Image;Investigation by Journal/Publisher;Investigation by Third Party;
- Date
- 3/28/2023 0:00
- Flagged by OpenAlex?
- Yes
Source: Retraction Watch, joined by DOI. OpenAlex records retraction as is_retracted, a boolean over a state space with at least four values, so it cannot express an expression of concern, a correction or a reinstatement — it reports them as false, which reads as “fine”.
Abstract
This study aimed to explore the effect of the TSLP–DC–OX40L pathway in asthma pathogenesis and airway inflammation in mice. For this, 65 male BALF/c mice were distributed among the control, asthma, immunoglobulin G (IgG) + asthma (IgG, 500 μg/500 μL, intratracheal injection of 50 μL each time), LY294002 (OX40L inhibitor) + asthma (intratracheal injection of 2 mg/kg LY294002), and anti-TSLP + asthma (intratracheal injection of 500 μg/500 μL TSLP antibody, 50 μL each time) groups. ELISA was applied to measure the serum levels of immunoglobulin E (IgE), ovalbumin (OVA)-sIgE, interleukin-4 (IL-4), IL-5, IL-13, and interferon-γ (IFN-γ); flow cytometry was employed to detect Treg cells and dendritic cell (DC) and lymphopoiesis. RT–qPCR and Western blot assays were used to measure the levels of TSLP, OX40L, T-bet, GATA-3, NF-κB, p38, and ERK. Treatment with LY294002 and anti-TSLP resulted in increases in the numbers of total cells, eosinophils, neutrophils, and lymphocytes in the bronchoalveolar lavage fluid; total serum levels of IgE, OVA-sIgE, IL-4, IL-5, and IL-13; levels of DC cells; lymphopoiesis; and levels of TSLP, OX40L, GATA-3, NF-κB, p38, and ERK, whereas there were decreases in the levels of IFN-γ and CD4 + CD25 + Treg cells; CD4 + Foxp3 + Treg cells; and T-bet. The TSLP–DC–OX40L pathway may contribute to asthma pathogenesis and airway inflammation by modulating the levels of CD4 + CD25 + Treg cells and inflammatory cytokines.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
The record
- Venue
- Biochemistry and Cell Biology
- Topic
- Asthma and respiratory diseases
- Field
- Medicine
- Canadian institutions
- —
- Funders
- —
- Keywords
- ImmunologyOvalbuminImmunoglobulin EFOXP3PathogenesisEosinophilAllergic inflammationIL-2 receptorMedicineInflammationBronchoalveolar lavageAntibodyAsthmaT cellImmune systemLungInternal medicine
- Has abstract in OpenAlex
- yes