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Record W2805514251 · doi:10.29173/spectrum35

Auto Sequencer: A DNA Sequence Alignment and Assembly Tool

2018· article· en· W2805514251 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
venuePublished in a venue whose home country is Canada.

Bibliographic record

VenueSpectrum · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsSequence assemblyDNA sequencingSequence (biology)Merge (version control)DNASequencing by ligationDNA sequencerComputer scienceDNA nanoball sequencingAlignment-free sequence analysisSoftwareHybrid genome assemblyComputational biologySequence analysisSequence logoConsensus sequenceReference genomeSequence alignmentAlgorithmBiologyGeneticsBase sequenceInformation retrievalProgramming languagePeptide sequenceGenomic libraryGene

Abstract

fetched live from OpenAlex

The process of determining the exact order of nucleotides in DNA is a crucial component of a wide varietyof research applications known as DNA sequencing. Over the last fifty years, several DNA sequencingtechnologies have been well characterized through their nature and the kind of output they provide. Evenwith significant advances in DNA sequencing technology, sequencing and assembly of large pieces ofDNA remains a complex task. It requires sequencing small reads of DNA at a time, and performing DNAsequence assembly to merge the individual pieces into a single contiguous sequence. DNA sequenceassembly, albeit tedious and time consuming, is a process in which short DNA sequence fragments aremerged into longer fragments in the attempt to reconstruct the original DNA sequence. This is usuallyachieved by manually identifying sequence overlaps between two reads before aligning them intoone contiguous sequence. Then, with the aid of online tools or software, this contiguous sequence istranslated into protein sequence. While this process may only take a few minutes, the complexity ofsequence translation and assembly can be driven by two major challenges: finding the most reasonableoverlap in sequences that may contain repeats or low quality regions, and outputting both nucleotideand protein sequence in an easy to use, comprehensive output. To facilitate this process, we introducean all-in-one tool: Auto Sequencer. This user-friendly tool can combine and translate raw DNA sequencefiles by finding the most reasonable overlap between them displaying outputs in flexible formats.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.110
Threshold uncertainty score0.459

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.014
GPT teacher head0.251
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it