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Record W2884308757 · doi:10.3892/ol.2018.9150

Detection of copy number variants and loss of heterozygosity from impure tumor samples using whole exome sequencing data

2018· article· en· W2884308757 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueOncology Letters · 2018
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCancer Genomics and Diagnostics
Canadian institutionsnot available
FundersBC Cancer AgencyNational Natural Science Foundation of China
KeywordsLoss of heterozygosityExome sequencingBiologyGeneticsExomeComputational biologyExonMutationAlleleGene

Abstract

fetched live from OpenAlex

Using whole-exome sequencing (WES) for the detection of chromosomal aberrations from tumor samples has become increasingly popular, as it is cost-effective and time efficient. However, factors which present in WES tumor samples, including diversity in exon size, batch effect and tumor impurity, can complicate the identification of somatic mutation in each region of the exon. To address these issues, the authors of the present study have developed a novel method, PECNV, for the detection of genomic copy number variants and loss of heterozygosity in WES datasets. PECNV combines normalized logarithm ratio of read counts (Log Ratio) and B allele frequency (BAF), and then employs expectation maximization (EM) algorithm to estimate parameters involved in the models. A comprehensive assessment of PECNV of PECNV was performed by analyzing simulated datasets contaminated with different normal cell proportion and eight real primary triple-negative breast cancer samples. PECNV demonstrated superior results compared with ExomeCNV and EXCAVATOR for the detection of genomic aberrations in WES data.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.009
Threshold uncertainty score0.391

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.034
GPT teacher head0.297
Teacher spread0.263 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it