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Record W2971641747 · doi:10.1038/s41593-020-0685-8

A community-based transcriptomics classification and nomenclature of neocortical cell types

2020· preprint· en· W2971641747 on OpenAlexafffund
Rafael Yuste, Michael Hawrylycz, Nadia Aalling, Argel Aguilar‐Valles, Detlev Arendt, Rubén Armañanzas, Giorgio A. Ascoli, Concha Bielza, Vahid Bokharaie, Tobias Bergmann, Irina Bystron, Marco Capogna, YoonJeung Chang, Ann M. Clemens, Christiaan P. J. de Kock, Javier DeFelipe, Sandra Dos Santos, Keagan Dunville, Dirk Feldmeyer, Richárd Fiáth, Gord Fishell, Angelica Foggetti, Xuefan Gao, Parviz Ghaderi, Natalia A. Goriounova, Onur Güntürkün, Vanessa Jane Hall, Moritz Helmstaedter, Suzana Herculano‐Houzel, Markus M. Hilscher, Hajime Hirase, Jens Hjerling‐Leffler, Rebecca D. Hodge, Rafiq Huda, Konstantin Khodosevich, Ole Kiehn, Henner Koch, Eric S. Kuebler, Malte Kühnemund, Pedro Larrañaga, Boudewijn P. F. Lelieveldt, Emma Louise Louth, Jan H. Lui, Huibert D. Mansvelder, Óscar Marín, Julio Martínez-Trujillo, Alok Nath Mohapatra, Hermany Munguba, Pavel Němec, Netanel Ofer, Ulrich Pfisterer, Samuel Pontes-Quero, Jean Rossier, Joshua R. Sanes, Richard H. Scheuermann, Péter Somogyi, Gábor Tamás, Andreas S. Tolias, Maria Antonietta Tosches, Miguel Turrero Garcίa, Christian Wozny, Thomas V. Wuttke, Hongkui Zeng, Ed S. Lein

Bibliographic record

VenueNature Neuroscience · 2020
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicSingle-cell and spatial transcriptomics
Canadian institutionsWestern UniversityKrembil FoundationCarleton University
FundersNational Institute of Neurological Disorders and StrokeNational Institute on AgingRobarts Research InstituteUniversity of California, San DiegoSzegedi TudományegyetemH. Lundbeck A/SNovo Nordisk FondenRWTH Aachen UniversityUniversity of HaifaLeids Universitair Medisch CentrumKarolinska InstitutetLundbeckfondenUniversity of OxfordVrije Universiteit AmsterdamChristian-Albrechts-Universität zu KielScience for Life LaboratoryBar-Ilan UniversityUniversiteit LeidenStockholms UniversitetBrainScope CompanyGeorg-August-Universität GöttingenNational Eye InstituteUniversidad Politécnica de MadridUniversity of EdinburghYork UniversityDepartment of Neurobiology, Harvard Medical SchoolEuropean Molecular Biology LaboratoryInstituo CajalSchulich School of Medicine and DentistryEberhard Karls Universität TübingenRIKENKing's College LondonVanderbilt UniversityUniverzita Karlova v PrazeMacquarie UniversityHarvard UniversityÉcole Polytechnique Fédérale de LausanneSorbonne UniversitéGeorge Mason UniversityMassachusetts Institute of TechnologyNational Institute of Mental HealthAarhus Universitet
KeywordsNeocortexCell typeProfiling (computer programming)Taxonomy (biology)NomenclatureNeuroscienceBiologyComputer scienceClassification schemeBiological classificationArtificial intelligenceData scienceCellEvolutionary biologyEcology

Abstract

fetched live from OpenAlex

To understand the function of cortical circuits, it is necessary to catalog their cellular diversity. Past attempts to do so using anatomical, physiological or molecular features of cortical cells have not resulted in a unified taxonomy of neuronal or glial cell types, partly due to limited data. Single-cell transcriptomics is enabling, for the first time, systematic high-throughput measurements of cortical cells and generation of datasets that hold the promise of being complete, accurate and permanent. Statistical analyses of these data reveal clusters that often correspond to cell types previously defined by morphological or physiological criteria and that appear conserved across cortical areas and species. To capitalize on these new methods, we propose the adoption of a transcriptome-based taxonomy of cell types for mammalian neocortex. This classification should be hierarchical and use a standardized nomenclature. It should be based on a probabilistic definition of a cell type and incorporate data from different approaches, developmental stages and species. A community-based classification and data aggregation model, such as a knowledge graph, could provide a common foundation for the study of cortical circuits. This community-based classification, nomenclature and data aggregation could serve as an example for cell type atlases in other parts of the body.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.004
metaresearch head score (Gemma)0.009
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.008
Threshold uncertainty score0.022

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0040.009
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0070.007
Science and technology studies0.0020.002
Scholarly communication0.0040.004
Open science0.0010.002
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.038
GPT teacher head0.277
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations25
Published2020
Admission routes2
Has abstractyes

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