Response to the Article “Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality”
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Having come across this article,1Buker S.M. Boriack-Sjodin P.A. Copeland R.A. Enzyme–Inhibitor Interactions and a Simple, Rapid Method for Determining Inhibition Modality.SLAS Discov. 2019; 24: 515-522Google Scholar I was quite disappointed in its one-sided biased endorsement of classical inhibition models. Whereas it may be inconceivable to many biochemists practicing in the field today that there is controversy surrounding the classical models of enzyme inhibition, one only needs to look at the propagation of subsequent inhibition models over the years to realize that the classical way of modeling segregates interactions into very strict predefined limitations. For example, traditional competitive inhibitors only decrease substrate affinity by linearly increasing the value of the KM with increasing inhibitor concentration. However, a mathematical model does not indicate mechanism; rather, it provides support for a hypothesis, which is why you may have allosteric effects that present as competitive, as outlined by the authors. Given that these equations do not really define specific interactions, there should be no point in advocating their use if there is a single equation that can model the data as well as or, in most cases, better than they can. The omission of this point from the article greatly reduces the overall usefulness of a review. By recognizing that the apparent inhibition term in the classical inhibition equations is an inversion of the inhibitor binding isotherm (eq 1),2Walsh R. Alternative Perspectives of Enzyme Kinetic Modeling.in: Ekinci D. Medicinal Chemistry and Drug Design. InTech, Rijeka, Croatia2012: 357-372Google Scholar one can directly relate changes in enzymatic activity to the fraction of the enzymatic population bound. Consequently, changes in enzymatic activity can be described through observation rather than strictly defined limits imposed by the classical equations (eq 2).3Walsh R. Martin E. Darvesh S. A Versatile Equation to Describe Reversible Enzyme Inhibition and Activation Kinetics: Modeling Beta-Galactosidase and Butyrylcholinesterase.Biochim. Biophys. Acta. 2007; 1770: 733-746Google Scholar 1+[I]Ki=1−[I][I]+Ki(1) v=[S][S]+(K1−ΔK1[X][X]+Kx)(V1−ΔV1[X][X]+Kx)(2) This equation has been tested against the classical equations with real data and has been found to allow for an equivalent or improved fit in all cases.3Walsh R. Martin E. Darvesh S. A Versatile Equation to Describe Reversible Enzyme Inhibition and Activation Kinetics: Modeling Beta-Galactosidase and Butyrylcholinesterase.Biochim. Biophys. Acta. 2007; 1770: 733-746Google Scholar, 4Walsh R. Comparing Enzyme Activity Modifier Equations through the Development of Global Data Fitting Templates in Excel.PeerJ. 2018; 6: e6082Google Scholar, 5Walsh R. A Reanalysis of Protein Tyrosine Phosphatases Inhibitory Studies Using the Unnatural Substrate Analogue p-Nitrophenyl Phosphate.Anal. Biochem. 2019; 572: 58-62Google Scholar The flexibility of this approach also allows the equation to be used to describe activators in addition to inhibitors. Any researchers can also quickly and easily test this approach with their own data and evaluate the fit against the classical models using a freely available Excel template.4Walsh R. Comparing Enzyme Activity Modifier Equations through the Development of Global Data Fitting Templates in Excel.PeerJ. 2018; 6: e6082Google Scholar Therefore, it is a disservice to the research community for the authors to recommend constraining mechanistic studies to classical inhibition equations with clear mathematical limitations based on mechanistic models the authors concede are not valid. Declaration of Conflicting Interests The author declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. The author received no financial support for the research, authorship, and/or publication of this article.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it