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Record W4399352026 · doi:10.1093/mictod/qaae024

Photosynthesis Got an Early Start

2024· article· en· W4399352026 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

aboutThe title or abstract carries a Canadian signal from the geographic lexicon.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueMicroscopy Today · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPhotosynthetic Processes and Mechanisms
Canadian institutionsnot available
Fundersnot available
KeywordsPhotosynthesisBiologyBotany

Abstract

fetched live from OpenAlex

Blue-green algae (cyanobacteria) have a bad reputation, but they played an essential role in the history of our planet. They produce toxins that can make people sick, but around 2.4 billion years ago (Giga annum or Ga) they played a key role in the oxygenation of the atmosphere and oceans, a period known as the Great Oxidation Event, and they are still important as primary producers of oxygen. Cyanobacteria are also the precursor of chloroplasts, organelles where oxygen-producing photosynthesis can take place within many living organisms, specifically those organisms that became plants. Although the production of oxygen is essential for most life as we know it, there has been no unambiguous evidence for thylakoids in the early fossil record until now. An elegant study by Catherine Demoulin, Yannick Lara, Alexandre Lambion, and Emmanuelle Javaux has provided such evidence and has moved the dawn of photosynthesis back 1.2 Ga! Light micrograph of Navifusa majensis, 1.75 billion years old, from the McDermott Formation, Australia. Length of organism = 110 µm. Photo copyright, E.J. Javaux. Demoulin et al. showed that analysis of the ultrastructure of fossils is an underappreciated tool for demonstrating chloroplast-like structures. They examined fossils from around the globe (Australia, Canada, and the Democratic Republic of the Congo) using transmission electron microscopy (TEM), specifically looking for thylakoids. Thylakoids are membrane-bound compartments where photosynthesis takes place in cyanobacteria and chloroplasts. Demoulin et al. searched for thylakoids in Navifusa majenis, microfossils that may be cyanobacteria. They isolated the carbonaceous microfossils from the mineralized matrix with a series of strong acid treatments and then embedded them in agarose. The specimens were dehydrated in a graded series of alcohol and embedded in resin. After sectioning, some sections were stained. Interestingly, Demoulin et al. observed no difference between stained and unstained specimens. Although this is difficult to explain, one factor is the microscope they used. The FEI Tecnai T12 provides suitable contrast in unstained sections. Another factor might be that during fossilization, the organic material was stained naturally by diverse elements from the sediment in which the cells were buried. The ultrastructure of unstained resin-embedded specimens showed a set of intracellular membranes with sharp, darker edges that appeared to be either parallel to the cell wall or locally contorted. Each membrane comprised one medium electron-dense layer surrounded by two electron-dense layers. These membranes are from 10–20 nm thick. Whereas these structures resemble basic units of chloroplasts, they are clearly not fully formed chloroplasts. They are probably thylakoids. Together with additional studies of the arrangement of intracellular membranes, this provides direct evidence for oxygen-producing photosynthesis. The value of using TEM to examine thin sections of microfossils was convincingly demonstrated by Demoulin et al. Their discovery of preserved thylakoids within N. majenis unambiguously establishes a minimum age of about 1.75 Ga for the divergence of cyanobacteria with and without thylakoids. Demoulin et al. predict that studies examining the ultrastructure of well-preserved fossils may more clearly define the organisms that created the Great Oxidation Event, leading to the metabolisms that have existed ever since then.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.135
Threshold uncertainty score0.685

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.271
Teacher spread0.262 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it