First detection of saffron dwarf virus, wheat dwarf virus, wheat dwarf virus‐associated alphasatellite and a new putative potyvirus species in saffron in Iran
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Saffron (Crocus sativus, Iridaceae), the most valuable spice in the world, is propagated vegetatively through corms and cultivated widely in Iran (Moradi et al., 2021). Several viruses have been reported in saffron in Iran: saffron latent virus, saffron yellow mosaic virus and beet western yellows virus (Parizad et al., 2018; Tavoosi et al., 2024; Haseli et al., 2024). During 2022, a total of 100 leaf tissue samples were collected from diseased (leaf curling, mosaics and mottling) (Figure 1) and asymptomatic saffron plants growing in cultivated fields in Fars and Tehran provinces in Iran (an area of 8,385 and 1,650 ha, respectively). The diseased and asymptomatic samples were pooled together into two groups of 50. Viral particles were enriched from the pooled samples using the virion-associated nucleic acid extraction protocol (Maclot et al., 2021). Libraries were prepared with the Illumina TruSeq PCR-Free preparation kit, and sequencing was performed on the NovaSeq 6000 platform with paired-end reads (2 × 150 bp). A total of 2,305,263 raw reads were obtained by high-throughput sequencing (HTS) and trimmed to 1,609,204 high-quality reads (with a quality score above 32) using BBDuK (version 38.84) with default parameters within Geneious Prime 2020.2. After de novo contig assembly using the SPAdes assembler (version 3.13.0), 6,608 contigs were generated. The tblastx comparison was conducted with a local database created using the viral refseq database (downloaded from NCBI, November 2021). The tentative viral contigs were extended by iterative mapping using Geneious assembler (custom sensitivity mode, with mismatch set according to the demarcation criteria of each virus). This resulted in three sequences with high nucleotide identity with known species: a sequence of 2,538 nt with 98% identity to wheat dwarf virus (WDV; GenBank Accession No. KU877917); a sequence of 2,726 nt with 96% identity to saffron dwarf virus (SaDV, BK067261); and a sequence of 1,486 nt with 89% identity to wheat dwarf virus-associated alphasatellite (WDVaA, PP445014). In addition, a contig of 9,548 nt (PQ740911) had limited homology (<71%) with various potyviruses, and the putative polyprotein (3,113 aa) shared 62% identity with Narcissus yellow stripe virus (BBE01234), suggesting that this sequence might correspond to a new potyvirus based on the demarcation threshold for members of the genus (Inoue-Nagata et al., 2022). To confirm the presence of the viruses detected in the pooled samples, PCR/RT-PCR assays were developed using novel specific primer pairs (Table 1). Amplified cDNA fragments of the expected size were sequenced bi-directionally, and showed 93–100% identity with the corresponding genome sequences obtained by HTS. These detections correspond to the first report of the presence of WDV (PQ740908), WDVaA (PQ740910) and SaDV (PQ740909) in saffron plants in Iran. The discovery of a putative new potyvirus (PQ740911), tentatively named Potyvirus crociranense (saffron Iran virus, SaIRV) will be further investigated. HTS data is available under Bioproject PRJNA1187297. Additional research is necessary to determine the prevalence and effects of these viruses on saffron crop. A. Dizadji, A. Golnaraghi and S. Massart contributed equally to this report. The authors would like to acknowledge the financial supports of Iran National Science Foundation (INSF, Project No. 4000914), Fond National de la Recherche Scientifique (FNRS, Belgium; Grant J.0149.20) and University of Tehran (73148924/6/19).
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.002 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it