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Record W4412108418 · doi:10.14573/altex.2501152s3

Chemical selection for the Thyroid Validation Study coordinated by EURL ECVAM and involving EU-NETVAL laboratories_suppl3

2025· article· en· W4412108418 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueALTEX · 2025
Typearticle
Languageen
FieldPhysics and Astronomy
TopicRadioactive Decay and Measurement Techniques
Canadian institutionsnot available
FundersRECETOX Přírodovědecké Fakulty Masarykovy UniverzityHealth CanadaUniversità di PisaUniversiteit AntwerpenUniversità di Catania
KeywordsThyroidSelection (genetic algorithm)ChemistryMedicineComputer scienceInternal medicineArtificial intelligence

Abstract

fetched live from OpenAlex

The aim of the Thyroid Validation Study, coordinated by EURL ECVAM and involving EU-NETVAL laboratories, was to validate selected non-animal methods for the identification of chemicals that can potentially disrupt the thyroid hormone system in humans. The validation study was organized in two parts: Part 1 was to assess method performance and develop standard operating procedures, where needed, and Part 2 was to assess the mechanistic relevance of the methods using a set of validation chemicals. This paper describes the stepwise process to select this validation set of chemicals, mainly based on extensive literature review and expert judgment elicitation to identify chemicals for which there was evidence to show their (lack of) ability to perturb the thyroid hormone signaling mechanisms or modes of action covered by the methods. A unique contribution of the study lies in its mechanistic coverage of molecular targets within the thyroid gland but also regulatory mechanisms in peripheral tissues, reflecting a multifaceted perspective on thyroid hormone action. The validation set consisted of 30 chemicals, providing a balanced representation across a broad chemical space and offering insights into the mechanistic relevance of the selected methods. Once validated, these methods will contribute to advancing the identification and evaluation of endocrine disruptors, informing regulatory decisions, and promoting alternative testing strategies. Plain language summaryThis manuscript provides important insights that will allow the progression of non-animal methods for thyroid hormone disruption and their use in the regulatory context. The selection of chemicals used in a validation study is of paramount importance and will impact on the overall performance of the methods being validated. The selection approach used is described in detail and provides a relevant and useful guide for possible future validation studies.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.348
Threshold uncertainty score0.307

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.008
GPT teacher head0.271
Teacher spread0.264 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it