Dexamethasone/β-cyclodextrin inclusion complex hydrogel for vital pulp therapy
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Targeting inflammation and mineralization responses in a controlled manner for vital pulp therapies is a challenging task. Here, we prepared dexamethasone-β-cyclodextrin (DEX/ICD) inclusion complex-loaded hydrogels for pulp-dentin complex regeneration. The formation of the DEX/ICD inclusion complex was verified using Fourier-transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Pristine dexamethasone (DEX) and DEX/ICD were added to methacrylated gelatin (GelMA) hydrogels at a concentration of 1%. Pristine GelMA serves as the control. Drug-loaded hydrogels were evaluated for mechanical properties (n = 10/group), swelling and enzymatic degradation (n = 5/group), and drug release (n = 3/group). Cell proliferation, cell morphology, and mineralization assays were conducted using SHEDs. Cell proliferation was assessed on days 1, 3, and 7 using the MTS assay (n = 6/group). Cell morphology was analyzed via fluorescence microscopy using phalloidin and DAPI. A mineralization assay was performed using Alizarin Red at 14 and 21 days (n = 4/group). One-way ANOVA and Tukey's (α = 0.05) post-hoc tests were applied for statistical analysis. FTIR and XRD data confirmed the formation of the DEX/ICD complex. No statistical differences were found in mechanical properties, swelling, and degradation after incorporating DEX or DEX/ICD. DEX/ICD-loaded hydrogels consistently released dexamethasone, peaking at day 14, and maintained stability until day 21. DEX-loaded GelMA release decreased after day 7. DEX/ICD-loaded GelMA exhibited higher cell proliferation than DEX-loaded GelMA on day 7 (p < 0.05), with no statistical differences observed for days 1 and 3 (p > 0.05). The hydrogels did not compromise cell morphology. DEX/ICD-loaded GelMA significantly enhanced mineralization potential (p < 0.05). The β-CD inclusion complex optimized dexamethasone delivery, and DEX/ICD-containing GelMA enhances the proliferation and mineralization capacity of dental stem cells. DEX/ICD-loaded GelMA is a promising injectable material for halting inflammation and could potentially induce mineralized tissue formation in pulp capping strategies.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it