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Record W4412573503 · doi:10.3897/imafungus.16.159349

Characterization of the mitochondrial genomes for Ophiostoma ips and related taxa from various geographic origins and related species: large intron-rich genomes and complex intron arrangements

2025· article· en· W4412573503 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueIMA Fungus · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicPlant and Fungal Interactions Research
Canadian institutionsUniversité de MontréalUniversity of Manitoba
FundersNatural Sciences and Engineering Research Council of Canada
KeywordsIntronBiologyGroup II intronGenomeGeneGeneticsRNA splicingEvolutionary biologyRNAComputational biology

Abstract

fetched live from OpenAlex

The Ophiostomatales are of economic concern, as many are blue-stain fungi and some are plant pathogens. The mitogenomes of members assigned to this order exhibit size polymorphism despite having highly conserved gene order, owing to the variable number of introns and intron insertion sites. In this work, eleven blue-stain fungi, including nine strains of Ophiostoma ips with a varied distribution across North America and New Zealand, were sequenced and compared with other members of the Ophiostomatales . A pan-mitogenome intron landscape has been prepared to demonstrate the distribution of the mobile genetic elements and to provide insight into the evolutionary dynamics of introns among members of this group of fungi. The size variation among these mitogenomes (from about 23.8 kb to 152 kb) shows high correlation to the presence and absence of introns. Examples of complex or nested introns composed of two or three intron modules have been observed in some O. ips strains. RNA-seq data suggests possible splicing pathways with regard to resolving these complex introns. Mitochondrial DNA and RNA data for O. ips provides the basis for future studies relating to gene annotation, alternative splicing, evolutionary intron dynamics, and taxonomic investigations for members of the Ophiostomatales .

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.877
Threshold uncertainty score0.454

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.254
Teacher spread0.244 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it