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Record W4413636273 · doi:10.1016/j.xplc.2025.101490

Antiviral RNA interference in plants: Increasing complexity and integration with other biological processes

2025· review· en· W4413636273 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.

Bibliographic record

VenuePlant Communications · 2025
Typereview
Languageen
FieldAgricultural and Biological Sciences
TopicPlant Virus Research Studies
Canadian institutionsAgriculture and Agri-Food Canada
FundersAgricultural Science and Technology Innovation ProgramNational Natural Science Foundation of China
KeywordsRNA interferenceComputational biologyBiologyRNAInterference (communication)VirologyGeneticsComputer scienceGeneTelecommunications

Abstract

fetched live from OpenAlex

RNA interference (RNAi, also known as RNA silencing) is one of the most important plant defense responses against viral invasion. Although major components of the RNAi pathway, steps leading to viral small interfering RNA biogenesis, and viral counterdefense strategies via RNAi suppressors have been well studied, the broader roles of RNAi in viral infection and seed transmission remain less thoroughly characterized. In particular, the increasing complexity of RNAi-associated mechanisms and their integration with other biological processes have not been comprehensively summarized. Increasing numbers of studies have identified non-canonical RNAi pathways, novel host factors involved in RNAi, and the possibility of small RNAs acting across kingdoms to modulate plant-virus-vector tritrophic interactions. In this review, we provide an overview of the roles of RNAi in plant viral infections and describe recent advances, with emphasis on the discoveries of novel positive and negative RNAi regulators, potential signaling pathways upstream and downstream of antiviral RNAi, and the prospects and challenges of double-stranded RNA applications, either expressed from transgenes or supplied exogenously via spraying. We also discuss how these findings reshape current views on antiviral RNAi, highlight remaining knowledge gaps, and examine how these advances influence plant-virus co-evolution while informing strategies for managing plant virus diseases and reducing their impact.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Other design · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.995
Threshold uncertainty score0.990

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.271
GPT teacher head0.370
Teacher spread0.099 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it