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Record W4415320742 · doi:10.1016/j.jbi.2025.104932

Towards a Biological Evaluation Framework for Oversampling (BEFO) gene expression data

2025· article· en· W4415320742 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

fundA Canadian funder is recorded on the work.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueJournal of Biomedical Informatics · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGene expression and cancer classification
Canadian institutionsnot available
FundersQueen's UniversityQueen's University Belfast
KeywordsOversamplingRanking (information retrieval)Random forestBiological dataRelevance (law)Synthetic dataPopulationClass (philosophy)

Abstract

fetched live from OpenAlex

Machine learning (ML) techniques are progressively being used in biomedical research to improve diagnostic and prognostic accuracy when used in conjunction with a clinician as a decision support system. However, many datasets used in biomedical research often suffer from severe class imbalance due to small population sizes, which causes machine learning models to become biased to majority class samples. Current oversampling methods primarily focus on balancing datasets without adequately validating the biological relevance of synthetic data, risking the clinical applicability of downstream model predictions. To address these shortcomings, we propose the Biological Evaluation Framework for Oversampling (BEFO) designed to ensure that synthetic gene expression samples accurately reflect the biological patterns present in original datasets. This innovation not only mitigates bias but enhances the trustworthiness of predictive models in clinical scenarios. We have developed a ranking method for synthetic samples based on this and evaluated each sample's inclusion based on its rank. This ranking method calculates the WGCNA gene co-expression clusters on the original dataset. Several random forests are constructed to assess the alignment of each synthetic sample to each cluster. Only synthetic samples more important than real samples are included in a study. The experimental results demonstrate that our proposed ML oversampling framework can improve the biological feasibility of oversampled datasets by an average of 11%, leading to improved classification performance by an average of 9% when compared against five state-of-the-art (SOTA) oversampling methods and ten classification algorithms across six real world gene expressions datasets. Thereby establishing a new standard for synthetic data evaluation in biomedical ML applications.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.002
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Methods · Consensus signal: none
Teacher disagreement score0.578
Threshold uncertainty score0.281

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.002
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0010.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.093
GPT teacher head0.402
Teacher spread0.309 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it