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Record W4416239741 · doi:10.1016/j.xpro.2025.104187

Protocol for creating a gene dictionary for organelle genomes using the Gene Dictionary Tool

2025· article· en· W4416239741 on OpenAlex

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

affAt least one author lists a Canadian institution in the pinned OpenAlex snapshot.
fundA Canadian funder is recorded on the work.

Bibliographic record

VenueSTAR Protocols · 2025
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicGenomics and Phylogenetic Studies
Canadian institutionsWestern University
FundersNatural Sciences and Engineering Research Council of CanadaFundação AraucáriaConselho Nacional de Desenvolvimento Científico e TecnológicoFundacion Araucaria
KeywordsPython (programming language)GenomeGeneProtocol (science)Interface (matter)User interfaceGene prediction

Abstract

fetched live from OpenAlex

Here, we present a protocol for creating a gene dictionary for fungal mitochondrial genomes using the Gene Dictionary Tool. Through a Python Command Line Interface (CLI), the user identifies what annotations are missing in the inputted dictionary. Via two Jupyter Notebooks, the user builds a gene dictionary based on attributes retrieved from inputted GFF3 files. The final output, a .gdict file, is findable, accessible, interoperable, and reusable (FAIR). This protocol can be adapted to create a gene dictionary for other genomes. • Protocol for creating a comprehensive and versionable gene dictionary across genomes • Guidance on how to use and implement the gdt Python library • Steps for the iterative creation of gene dictionaries for organelle genomes • Instructions on how to process genome features with poor identifying information Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol for creating a gene dictionary for fungal mitochondrial genomes using the Gene Dictionary Tool. Through a Python command line interface, the user identifies what annotations are missing in the inputted dictionary. Via two Jupyter Notebooks, the user builds a gene dictionary based on attributes retrieved from inputted GFF3 files. The final output, a .gdict file, is findable, accessible, interoperable, and reusable (FAIR). This protocol can be adapted to create a gene dictionary for other genomes.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Protocol · Consensus signal: Protocol
Teacher disagreement score0.556
Threshold uncertainty score0.678

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0010.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.350
Teacher spread0.311 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it