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Size Matters: A Biophysical Perspective on Biomolecular Condensates in Bacteria

2025· review· en· W4417287106 on OpenAlexaff
Lydia Hodgins, Baljyot Singh Parmar, Rodrigo Reyes‐Lamothe, Stephanie C. Weber

Bibliographic record

VenueAnnual Review of Biophysics · 2025
Typereview
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicBacterial Genetics and Biotechnology
Canadian institutionsMcGill University
Fundersnot available
KeywordsBacteriaNucleationCompartment (ship)Synthetic biologyPerspective (graphical)Bacterial cell structure

Abstract

fetched live from OpenAlex

Bacteria are unicellular organisms that typically lack membrane-bound organelles. Nevertheless, they are not merely "bags of enzymes" and instead use alternate mechanisms to organize their components in space and time. Biomolecular condensates are a newly described class of membraneless compartment that organizes cellular functions in bacteria. In this review, we cover key biophysical features of bacterial cells and discuss how their finite size and crowded interior may affect condensate nucleation and stability. Next, we describe three examples of endogenous condensates, highlighting the molecular components driving their formation and the functional roles they may play in cells. Finally, we provide an overview of current and prospective tools to study and manipulate both endogenous and synthetic condensates alike. Overall, bacterial condensates present a fascinating system to explore open questions that span the disciplines of biophysics, molecular and cell biology, and bioengineering.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Not applicable · Consensus signal: none
GenreCandidate signal: Review · Consensus signal: Review
Teacher disagreement score0.003
Threshold uncertainty score0.009

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.001
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0020.002
Science and technology studies0.0000.002
Scholarly communication0.0010.003
Open science0.0010.001
Research integrity0.0020.003
Insufficient payload (model declined to judge)0.0030.003

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.313
Teacher spread0.304 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designNot applicable
Domainnot available
GenreReview

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2025
Admission routes1
Has abstractyes

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