Rhodosporidium Toruloides as Robust Yeasts for Advanced Biofuel Production Using Wood Hydrolysate
Why this work is in the frame
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Bibliographic record
Abstract
In response to increasing global energy demand as well as greenhouse emissions from petroleum fuels, sustainable and renewable sources have been intensely researched to produce biofuel. For instance, microbial lipids have been recognized as a potential feedstock for biofuel production due to their similarities with vegetable oils in terms of fatty acids. Typically, microorganisms capable of accumulating more than 20% lipids are known as oleaginous microorganisms. These microorganisms can thrive on various renewable substrates and biochemically convert excess carbon into lipids. One such example of a renewable substrate is lignocellulosic biomass, which produces hydrolysate containing hexoses and pentoses sugars upon pre-treatment and saccharification, and thus could be employed as a potential substrate for microbial lipids. However, wood hydrolysate presents several challenges such as low consumption of pentose sugars, and the presence of microbial growth inhibitors such as furans, organic acids and phenols. In this sense, Rhodosporidium toruloides, an oleaginous yeast, could be employed to produce lipids due to its ability to accumulate 50-70% of lipids, consume C5 sugars, and tolerate inhibitors. Thus, the present thesis explores the ability of R. toruloides-1588 to thrive on undetoxified hydrolysate derived from forestry residues (hardwood and softwood sawdust) and accumulate lipids. Additionally, several strategies were employed to increase the lipid titer such as carbon and nitrogen ratios, fed-batch fermentation, and carbohydrate supplementation such as crude glycerol, which resulted in maximum lipid accumulation of 56.3% (w/w) along with more than 90% consumption of carbohydrates. A life cycle assessment has been also performed to identify the hotspots in terms of energy consumption, greenhouse gas emission, and waste produced during the lipid production process. Lastly, the strain was accessed for its ability to thrive on microbial growth inhibitor such as furfural and use it as an energy source. Based on the above findings, the current dissertation concludes that R. toruloides-1588 can thrive on undetoxified wood hydrolysate, accumulate lipids that can serve as a feedstock for biofuel production and provide aid in the further development of biorefinery industries.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.001 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.002 |
| Science and technology studies | 0.001 | 0.001 |
| Scholarly communication | 0.000 | 0.002 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.001 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it