Characterization of innate immune response to «Nicotiana benthamiana»-derived Influenza H5 virus-like particles
Why is this work in the frame?
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
No Canadian affiliation. An affiliation-only frame — the usual design — would never have seen this work. It is one of the works that make the case for inverting the frame.
The three-model screen
all 1,000 screened works →All three models called this out of scope.
Immunology study of plant-derived influenza virus-like particles.
The study examines immune responses to a vaccine candidate, not research itself.
Vaccine immunology of plant-derived influenza VLPs, domain biomedical science.
Abstract
Current influenza vaccine manufacturing processes using chicken-embryonated egg technology is a time-consuming and laborious process, and is currently the major drawback in counteracting pandemic influenza strain. One solution to that problem is the use of plants to generate vaccine antigen. Virus-like particles (VLP), produced from the tobacco plant Nicotiana benthamiana, represent a cost-effective, alternative platform for influenza vaccine production. Previous studies have shown that the immunization with VLP expressing the hemagglutinin (HA) protein from influenza virus H5N1 (H5-VLP) produced in N. benthamianainduce protective immunity against challenge of cross-clade virus in mice and ferrets. In this study, we used human peripheral blood mononuclear cells (PBMC) to characterize the innate immune response to plant-derived influenza H5-VLP ex vivo. We successfully demonstrate the mitogenic property of H5-VLP on PBMC ex vivo. Furthermore, we detect up-regulation of activation marker in B cells and NK cells, and some T cells. Cytokine profile of the supernatant from VLP-stimulated sample suggests that inflammatory response dominates the innate immunity within first 48 hours and is produced by CD14+ monocytes. Our study demonstrates that tobacco plant-derived influenza VLP are capable of generating innate immune responses in naïve human PBMC, helping us to better understand the immunostimulatory nature of this potential vaccine candidate.
Stored with the screening record, where it is evidence for the labels above.
The record
- Venue
- Library and Archives Canada (Government of Canada)
- Topic
- Magnetic confinement fusion research
- Field
- Physics and Astronomy
- Canadian institutions
- —
- Funders
- —
- Keywords
- NucleofectionFusible alloyTSG101ProteogenomicsHyporeflexiaLiquation
- Has abstract in OpenAlex
- yes