Microbial diversity of active layer soil from the Canadian high Arctic
Notice bibliographique
Résumé
Active layer is the surface layer of permafrost, which is an extraterrestrial analogue. The repeats of thawing and freezing make active layer an extreme environment for microorganisms. The bacterial and archaeal diversity in an active layer soil from the Canadian high Arctic was analyzed by using a high resolution pyrosequencing analysis. Since last few years, DNA sequencing-based microbial community studies have been widely propagated by the invention of pyrosequencing which produce millions of sequences in a single run (Ronaghi, 2001). This can exclude the time-consuming step such as constructing clone libraries and generates millions of sequencing in a single run. Therefore, tremendous sequence information in a sample can make be visualized the deep insights of microbial population. Total 7,796 bacterial reads for 40 phyla and 245 archaeal reads for 4 phyla were collected, reflecting the high diversity of bacteria. Much higher diversity in bacteria than in archaea was confirmed from the statistical analysis. Among these 40 phyla, 22 phyla were assigned as well-known phyla such as Proteobacteria and Bacteroidetes, whereas 18 phyla assigned as candidate phyla such as TM7, OD1. Proteobacteria (most were from the class Alphaproteobactera and Betaproteobacteria) and Bacteroidetes encompassed the majority of sequences with 67.8%. This is shown a good agreement of soil microbial communities in four different types of soils from Brazil, Florida, Illinois and Canada (Roesch et al., 2007), despite of targeting different region of 16S rRNA gene with this study. A good agreement was found that phylum Proteobacteria was the most dominant with 27.6 – 58.2% in all environments. Bacteroidetes was detected with 30.0% in case of Canadian permafrost, however, around 10% in other environments. The majority of the sequences from rhizosphere soil on Antarctica were affiliated to the Actinobacteria (28.7%) that were typically dominated in soil environment. In case of Archaea, relatively simple community composition was recognized. Euryarchaeota (51.4%) and Thaumarchaeota (46.1%) were major constituents of the archaeal community. The relatives of major species in this study were also isolated from various environments, mainly from freshwater lakes and also from alpine or polar habitats. Taken together, our results indicated that analysis of 16S rRNA gene derived from bar-coded pyrosequencing reads provide the necessary resolution and discrimination to be useful as a next generation platform for microbial diversity study in the Arctic active layer soil.
Récupéré en direct depuis OpenAlex et désinversé. Les résumés ne sont pas conservés dans cette base de données : les index inversés représentent 8,6 Go des 9,3 Go de texte de la base, et le serveur dispose de 13 Go libres.
Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,000 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,001 | 0,002 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,001 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,014 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».