29 HEALTH AND REPRODUCTIVE PROFILES OF NUCLEAR TRANSFER GOATS PRODUCING THE MSP1-42 MALARIA ANTIGEN
Notice bibliographique
Résumé
Somatic cell nuclear transfer (NT) using transfected primary cells is an efficient approach for the generation of transgenic large animals. However, abnormalities associated with the NT process could compromise the usefulness of cloned animals. In order to evaluate whether cloned animals could be used for the production of recombinant therapeutic proteins in milk, we examined the health, reproductive performance and milk production of 4 transgenic does derived from somatic cell NT. Two versions of the MSP1-42 transgene encoding either a glycosylated or a non-glycosylated MSP1-42 protein were constructed. Following somatic cell NT, 4 healthy transgenic does were born (Chen LH et al., 2002 Theriogenology 57, 777 abst). Does 1A and 2A were obtained from the same cell line carrying the glycosylated MSP1-42 transgene, whereas 3A and 4A were from a cell line carrying the non-glycosylated transgene. Health and growth of these NT animals was monitored with the supervision of a veterinarian and compared to age-matched control dairy does. All animals were bred naturally and, except for one control doe, delivered healthy kids. The 4 NT does produced a total of 9 kids of which 8 were live and one was stillborn. The control does delivered 5 kids, one fetus being lost during early pregnancy. Three of the eight kids from the NT animals were transgenic; all kids were normal and healthy. All does were milked after parturition, and recombinant protein expression in the milk of NT does was evaluated. There were no differences in birth and weaning weights or fertility between NT and control animals. The NT does expressing the glycosylated antigen (1A, 2A) produced only small amounts of milk at induction and postpartum. The reduced milk yield for that line is possibly caused by the expression of the transgene. However, normal lactations were achieved with the 2 does expressing the non-glycosylated antigen (3A, 4A), each producing in 100 days enough MSP1-42 protein to generate 5 million doses of the Malaria vaccine. These data demonstrate that transgenic goats produced by somatic cell NT can be used successfully for the recombinant production of therapeutic proteins.
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Comment cette classification a été obtenuedéplier
Prédiction distillée sur la base complète
Imitation des enseignantsNi prévalence calibrée, ni vérité terrain. Validation humaine à venir. Apprise à partir de 10 348 étiquettes directes de Codex et de 10 348 étiquettes directes de Gemma. Le mode candidate est l'union des têtes enseignantes seuillées; le consensus est leur intersection. Ces sorties portent le statut machine_predicted_unvalidated et ne sont ni des étiquettes humaines ni des étiquettes directes de modèles de pointe.
Scores Codex et Gemma par catégorie
| Catégorie | Codex | Gemma |
|---|---|---|
| Métarecherche | 0,001 | 0,000 |
| Méta-épidémiologie (sens strict) | 0,000 | 0,000 |
| Méta-épidémiologie (sens large) | 0,000 | 0,000 |
| Bibliométrie | 0,000 | 0,000 |
| Études des sciences et des technologies | 0,000 | 0,000 |
| Communication savante | 0,000 | 0,000 |
| Science ouverte | 0,000 | 0,000 |
| Intégrité de la recherche | 0,000 | 0,000 |
| Charge utile insuffisante (le modèle a refusé de juger) | 0,000 | 0,000 |
Scores machine (provisoires)
Les deux têtes enseignantes du modèle étudiant, lues sur ce travail. Un score ordonne la base pour la relecture; il n'affirme jamais une catégorie, et le statut de validation accompagne chaque rangée tel quel.
Scores de référence d'un modèle non mature (critères de maturité non atteints, 7 itérations). Un score ordonne; il n'affirme jamais une catégorie.
score_only:v0-immature-baseline · tel quel depuis la passe de notation : score_only signifie que le nombre peut ordonner les travaux, et qu'aucune étiquette de catégorie n'en découleClassification
machine, non validéePrédiction automatique; un appel candidat d’une seule tête enseignante, pas un consensus.
Le détail, modèle par modèle et score par score, se trouve en fin de page sous « Comment cette classification a été obtenue ».